Sequences from confirmation amplicon


Confirmation sequences for line 516B05 and hit At4g33950 are shown.
Line ID 516B05
Locus At4g33950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:16272491
Primers used for PCR RU81 - 8474, show primer details
   
Sequence Name 89-K027491-022-516-B05C-8409
GenBank Accession LN514905 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K027491-022-516-B05C-8409
AAATTGGTGATCCATCATACTCTTTGCTTGTCTCTCATCAATCTCGCAACTCCAAAATTA
CCGGAGCCAATATCCTTGACGAGTTCATACCTATCACTATCGTGCATAATCGGCAAATCC
ATTGGACCACTCACTGCTGGTCGATCCATTTTCTCCCCCTCTTTCCTCTGTCTCTTTGCG
ACTTCAATCTCTGTGTCAAGAAGATTTCTTTTTTGGGAGACAATATCTAACGACACCAAG
ACATCAGAGGAAATGAACAACGGTGTGACGTGGATGAATTTGAGCCTTTAAGATGAGAAA
AGGAGTATTGGGTGATCAAGTTATGGTTGTGAGAAAGAGACGTTTTCAGAGACCCGGGAA
AGTA
   
Sequence Name 90-K027491-022-516-B05C-RU81
GenBank Accession LN514991 [GenBank]
Sequencing primer gene specific primer, RU81: 5'-AGATACTTCACGGGTCTCTGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>90-K027491-022-516-B05C-RU81
CCAATACTCCTTTTCTATCTTAAGGGCTCAAATTCATCCACGNTCACATCGATTGTTCAT
TTCCTCTGATGTCTTGGTGTCGTTAGATATTGTCTCCCAAAAAAGAAATCTTCTTGACAC
AGAGATTGAAGTCGCAAAGAGACAGAGGAAAGAGGGGGAGAAAATGGATCGACCAGCAGT
GAGTGGTCCAATGGATTTGCCGATTATGCACGATAGTGATAGGTATGAACTCGTCAAGGA
TATTGGCTCCGGTAATTTTGGAGTTGCGAGATTGATGAGAGACAAGCAAAGAGTATGATG
GATCACCAATTTTACATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTAC

Last Updated on 06.12.2018 16:11