Sequences from confirmation amplicon


Confirmation sequences for line 516E01 and hit At5g53550 are shown.
Line ID 516E01
Locus At5g53550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:21757712
Primers used for PCR JL16 - 8474, show primer details
   
Sequence Name 61-K032419-022-516-E01C-8409
GenBank Accession LN510433 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K032419-022-516-E01C-8409
TTACAATTAAAAAGTATCTTGATGAATTGGTAAAGCCCGTCTCCGAGGATCAATGAGATT
GATATAAACACCTAAAGAGTTATGAACAAATTAAGAACGAAAGTACGCGAAAGAAAGGGC
GGAGTTTATTGTAGAGTACTAGTGTTTTTTGTATGGAACCTTGTAACCATTGAGACTCTT
CATGCTGTTTTCAGGAAGAGTTGATGGGAACCAATCTCCTTTAAGACCTTTAATGAGTGG
CCACATGATTCCCCAAGACAGAACCGCGCCAAAAAGCAAAGATATATTGACAATATGGGG
ACAGATCATTCCTGCTCCAACGTATGTCATGCTAAAGTCGAAGTAGAATCTGTCAAGTTT
TTTCAAGATCATCAAGATTCTGTTTCTATCTGTTGATATATAAACAGTCAATAGAACTAA
GAATAATACTCACGTGTTCTTCAAAGCTTCTAACCCGAAAGTTGGAAACTGAATGAAACC
GCACTCTGTACCACCAGAGAAGAACCATG
   
Sequence Name 61-K032419-022-516-E01C-JL16
GenBank Accession LN510434 [GenBank]
Sequencing primer gene specific primer, JL16: 5'-CTTTCTTCCAATGGTTCTTCTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K032419-022-516-E01C-JL16
TTTCACTTTCGGGTAGAAGCTTTGAAGAACACGTGAGTATTATTCTTAGTTCTATTGACT
GTTTATATATCAACAGATAGAAACAGAATCTTGATGATCTTGAAAAAACTTGACAGATTC
TACTTCGACTTTAGCATGACATACGTTGGAGCAGGAATGATCTGTCCCCATATTGTCAAT
ATATCTTTGCTTTTTGGCGCGGTTCTGTCTTGGGGAATCATGTGGCCACTCATTAAAGGT
CTTAAAGGAGATTGGTTCCCATCAACTCTTCCTGAAAACAGCATGAAGAGTCTCAATGGT
TACAAGGTTCCATACAAAAAACACTAGTACTCTACAATAAACTCCGCCCTTTCTTTCGCG
TACTTTCGTTCTTAATTTGTTCATAACTCTTTAGGTGTTTATATCAATCTCATTGATCCT
CGGAGACGGGCTTTACCAATTCATCAAGATACTTTTTAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAG
TACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACA
AATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
Primers used for PCR FP67 - 3144, show primer details
   
Sequence Name 10-K033040-022-516-E01C-FP67
GenBank Accession LN490546 [GenBank]
Sequencing primer gene specific primer, FP67: 5'-ACTGAAACCTAACGATGGAGCTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K033040-022-516-E01C-FP67
ATTTCGCTCGGGGAACATTATAGGGATCGCGATGATCGAGACAACAGAGAACGCTGCGTA
TCCTACTGCTGCAACCCATAATGGAATGCTGTCTCTTACAAAGATCTCATCTCTTTTAAG
ATCTGCAATAGATTGCTTATCTTTCTCCGAATCTGCGATTTACGTTTGAACATCAGATAA
TACATTATTGAAATGACGATTTTTCTATAGTTTTTTTGGTGTATACTTACTAGATTTCCC
AGAGTTGCGATTGTTTAACTTGACGTACATGTTTATTCCCCGGGTACCCTGTCCTCTCCA
AATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGT
CATCCCTTACGTCAGTGGAGATATCACATCAATCCACTTGCTTTGAAGACGTGGTTGGAA
CGTCTTCTTTTTCCACGATGCTCCTCGTGGGTGGGG
   
Sequence Name 42-K033040-022-516-E01C-3144
GenBank Accession LN504363 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K033040-022-516-E01C-3144
TCCACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACA
GGGTACCCGGGGAATAAACATGTACGTCAAGTTAAACAATCGCAACTCTGGGAAATCTAG
TAAGTATACACCAAAAAAACTATAGAAAAATCGTCATTTCAATAATGTATTATCTGATGT
TCAAACGTAAATCGCAGATTCGGAGAAAGATAAGCAATCTATTGCAGATCTTAAAAGAGA
TGAGATCTTTGTAAGAGACAGCATTCCATTATGGGTTGCAGCAGTAGGATACGCAGCGTT
CTCTGTTGTCTCGATCATCGCGATCCCTATAATGTTCCCCGAGCTGAAATGGTACTTCAT
AGTCGTAGCTTACATGTTAGCTCCATCGTAGGGTTTCA

Last Updated on 06.12.2018 16:11