Sequences from confirmation amplicon


Confirmation sequences for line 516F12 and hit At1g03970 are shown.
Line ID 516F12
Locus At1g03970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1018637
Primers used for PCR BF59 - 8474, show primer details
   
Sequence Name 26-K030141-022-516-F12C-8409
GenBank Accession LN497791 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K030141-022-516-F12C-8409
CATTTCATTGAATATATCCATTTACATCGATTTCATCTGAAGCTCCCTCGTCCATTTCTG
CTTTCGCTAAGAAATCCTCAAGTGTCATTATATCTTCTGGTTCTTCTTCCTTCATCATGA
TCGTCTTTTGCTCTCCAGATACAATCTCTTTCCAAACATCGTCAACACTCTTTCCGTTGT
TAACGCTGTTCCGATCTCCGATACTCCGATCAACATCAATCGCCATCTCGAAGGGCTTTG
AATCAGACGCGAAAGTGTAATCGTTGACTCCTCCGCCGTAAGCGAAACTGATTCTGGAGT
GATCGGCGTGAGGATTTGGTCGGAGATGGTGCGATGATCTTATAGAAGGGGAAGATGAAG
CAGAAGAAGAATTACGGCGAGACAAGTCGGAATTGGAAGAAGACATCAACTTGAAGGACG
CCATTTCCGGAGCTCACTGGAATTGGAACGAATTTTTATCTATCTGGGTTTTGTCAAAGA
GAAAGAACAATCGGAAATGGTGGATTTTCTTCGAACCAGAGATCGAGAGAGATCTTTCTA
GGGTTATCTCTAGTGTTTTGCAATGTAACCACGCGCTTACTCTCTATATACAAGTTTAAT
TAACTAATCA
   
Sequence Name 26-K030141-022-516-F12C-BF59
GenBank Accession LN497792 [GenBank]
Sequencing primer gene specific primer, BF59: 5'-GTTTAATGTGCGCGTGATTAGTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K030141-022-516-F12C-BF59
TAGCGCGTGGTTCATTGCAAACACTAGAGATAACCCTAGAAAGATCTCTCTCGATCTCTG
GTTCGAAGAAAATCCACCATTTCCGATTGTTCTTTCTCTTTGACAAAACCCAGATAGATA
AAAATTCGTTCCAATTCCAGTGAGCTCCGGAAATGGCGTCCTTCAAGTTGATGTCTTCTT
CCAATTCCGACTTGTCTCGCCGTAATTCTTCTTCTGCTTCATCTTCCCCTTCTATAAGAT
CATCGCACCATCTCCGACCAAATCCTCACGCCGATCACTCCAGAATCAGTTTCGCTTACG
GCGGAGGAGTCAACGATTACACTTTCGCGTCTGATTCAAAGCCCTTCGAGATGGCGATTG
ATGTTGATCGGAGTATCGGAGATCGGAACAGCGTTAACAACGGAAAGAGTGTTGACGATG
TTTGGAAAGAGATTGTATCTGGAGAGCAAAAGACGATCATGATGAAGGAAGAAGAACCAG
AAGATATAATGACACTTGAGGATTTCTTAGCGAAAGCAGAAATGGACGAGGGAGCTTCAG
ATGAAATCGATGTAAATGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATNTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11