Sequences from confirmation amplicon


Confirmation sequences for line 517C05 and hit At1g18140 are shown.
Line ID 517C05
Locus At1g18140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6240731
Primers used for PCR 22hz - 8474, show primer details
   
Sequence Name 29-K112562-0022-517-C05C-22hz
GenBank Accession LN499359 [GenBank]
Sequencing primer gene specific primer, 22hz: 5'-GAATGGAAGAAGGTAACTCGATTG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K112562-0022-517-C05C-22hz
GACATATCCAGGACCGACCGTGGCAGTACACGAAGGTGACATCGTAGAAATCAAAGTGAC
TAACCGGATTGCTCACAATACAACTATTCATTGGTAACACTCGTGCATGCATAATTCAAC
CACTTTTAACATCTTGTTTTAGTTCTTTCTGTGAGTTTATTTATCGTTTTTATATTTTTG
GGGGTTTGTGAGCAGGCATGGGTTAAGGCAATACCGGACCGGTTGGGCTGATGGACCGGC
TTACATAACGCAATGTCCGATAAGATCGAAACAGTAATTCAAAGAAAAAGAACAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCA
   
Sequence Name 05-K112561-0022-517-C05C-8409
GenBank Accession LN488425 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K112561-0022-517-C05C-8409
CATTTCATTGAATATATCCTGTTCTTTTTCTTTGAATTACTGTTTCGATCTTATCGGACA
TTGCGTTATGTAAGCCGGTCCATCAGCCCAACCGGTCCGGTATTGCCTTAACCCATGCCT
GCTCACAAACCCCCAAAAATATAAAAACGATAAATAAACTCACAGAAAGAACTAAAACAA
GATGTTAAAAGTGGTTGAATTATGCATGCACGAGTGTTACCAATGAATAGTTGTATTGTG
AGCAATCCGGTTAGTCACTTTGATTTCTACGATGTCACCTTCGTGTACTGCCACGGTCGG
TCCTGGATATTGTCCGTTCACCGTTAGAAGTTGTTTGGTGTGGCACAATCGAGTTACCTT
CTTCCATT

Last Updated on 06.12.2018 16:11