Sequences from confirmation amplicon


Confirmation sequences for line 517E09 and hit At3g03550 are shown.
Line ID 517E09
Locus At3g03550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:851296
Primers used for PCR 21pq - 8474, show primer details
   
Sequence Name 47-K115259-0022-517-E09C-21pq
GenBank Accession LN506289 [GenBank]
Sequencing primer gene specific primer, 21pq: 5'-GTATTAACACCATCATGCAAAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K115259-0022-517-E09C-21pq
TCTCCTCTATAATACCAATTAACTCGAAAAAAAAAATAGACACTTACCAGACAAAAGCAG
AGCCCAAAGAAAAAGGAGAGAGACACTTGACACCTCCATCTTCTTCCTTGCTCTCTCTCT
CTATCTTTCTCCAACAATCAAATAATGGGTTCAACAGGAAACCCTAATCCATGGGGTACA
ACATACGATTCATACAGAGACTGTTCTCAAGGAGTATGCAGTGTCTATTGCCCTCAATGG
TGTTACGTCATCTTCCCTCCTCCTCCTTCCTTCTATCTCGACGACGAAGACGACTCTTCC
TCCTCTGATTTCTCTCCTCTCCTCATCGCTCTCATCGGAATCCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCGCAGCGTTATTATA
   
Sequence Name 07-K115258-0022-517-E09C-8409
GenBank Accession LN489400 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K115258-0022-517-E09C-8409
GAATCAGAGGAGGAAGAGTCGTCTTCGTCGTCGAGATAGAAGGAAGGAGGAGGAGGGAAG
ATGACGTAACACCATTGAGGGCAATAGACACTGCATACTCCTTGAGAACAGTCTCTGTAT
GAATCGTATGTTGTACCCCATGGATTAGGGTTTCCTGTTGAACCCATTATTTGATTGTTG
GAGAAAGATAGAGAGAGAGAGCAAGGAAGAAGATGGAGGTGTCAAGTGTCTCTCTCCTTT
TTCTTTGGGCTCTGCTTTTGTCTGGTAAGTGTCTATTTTTTTATTTCGAGTTAATTGGTA
TTATTAGAGGAGATAATGAATAAATATATATGTTCATGAAAGCTTTTGCATGATGGTGTT
AATA

Last Updated on 06.12.2018 16:11