Sequences from confirmation amplicon


Confirmation sequences for line 517G02 and hit At2g46020 are shown.
Line ID 517G02
Locus At2g46020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18931569
Primers used for PCR 17po - 8474, show primer details
   
Sequence Name 35-K115331-0022-517-G02C-17po
GenBank Accession LN501949 [GenBank]
Sequencing primer gene specific primer, 17po: 5'-GGTTTTCACATGAGGTTTGATTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K115331-0022-517-G02C-17po
GATTCTTTGCTGTTTGATTGAAAGCCATTGGATTTAAGGTGATCAAAATTTTCGCAGGTG
AGATCTGAAGCAAAGAAGGTTCACAATCTGTTCTTTGATCTATTAAAGATGTCTTTCCCA
GACACAGATTTCCGGGAAGCAAGGAATGCTCTTTCCTTTTCTGGCTCAGCCCCAACTTTG
GTGTCTACACCAACCCCAAGAGGAGCAGGAATTAGCCAAGGCAAGAGGCAAAAGTTAGTA
AACGAGCCGGAAACCGAGCCAAGCTCTCCTCAGAGATCTCAACAACGTGAGAACTCAAGG
ATCAGAGTACAGATACCGCAGAAAGAAACAAAGCTTGGGGGGACAACAAGCCACACTGAT
GAATCCCCAATATTAGCTCATCCAGGGGAATTAGTAATCTGCAAGAAAAAGAGGAAAGAC
CGAGAGAAGTCTGGTCCGAAAACCCGAACTGGTGGCTCCAGCAGCCCAGTTTCACCACCT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 11-K115330-0022-517-G02C-8409
GenBank Accession LN491250 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K115330-0022-517-G02C-8409
TTCATTGAGGTGGTGAACTGGGCTGCTGGAGCCACCAGTTCGGGTTTTCGGACCAGACTT
CTCTCGGTCTTTCCTCTTTTTCTTGCAGATTACTAATTCCCCTGGATGAGCTAATATTGG
GGATTCATCAGTGTGGCTTGTTGTCCCCCCAAGCTTTGTTTCTTTCTGCGGTATCTGTAC
TCTGATCCTTGAGTTCTCACGTTGTTGAGATCTCTGAGGAGAGCTTGGCTCGGTTTCCGG
CTCGTTTACTAACTTTTGCCTCTTGCCTTGGCTAATTCCTGCTCCTCTTGGGGTTGGTGT
AGACACCAAAGTTGGGGCTGAGCCAGAAAAGGAAAGAGCATTCCTTGCTTCCCGGAAATC
TGTGTCTGGGAAAGACATCTTTAATAGATCAAAGAACAGATTGTGAACCTTCTTTGCTTC
AGATCTCACCTGCGAAAATTTTGATCACCTTTAAATCCAATTGGCTTTTCAATCAAACAG
CAAAAGGAATTCATTTTTATTTACAATGCANAAATCAAACCTCAAGTGAAAAC

Last Updated on 06.12.2018 16:11