Sequences from confirmation amplicon


Confirmation sequences for line 518D05 and hit At3g51450 are shown.
Line ID 518D05
Locus At3g51450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19091510
Primers used for PCR NH62 - 8474, show primer details
   
Sequence Name 04-K102660-0022-518-D05C-8409
GenBank Accession LN487868 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K102660-0022-518-D05C-8409
GAGCCATTTACAATTGAATACTTCTTTCCGTCACCGCTTATATTCAACAGTCCCTATACA
TAACAACAAAGTCAAACCTAAGATTGTGATTTTTCAAAATTCGATTTCAAAACCCCAACC
TTGTGTACGTCTGCGACAATGACTTCGCCGTGGATTCCGAACGCGATTCCGAGCGGTCTA
CCACCAGTATTGACCCAATCCTCAACGACTGAGTCATTAACCGAGTCGGCGACCTTGACT
CGTTTCACCCATCCATCAACACAACCAGTGTAGATGAGATTAGATTCCTTATGGTAGGCG
ATATCTTCGGGTATATTG
   
Sequence Name 23-K102661-0022-518-D05C-NH62
GenBank Accession LN496508 [GenBank]
Sequencing primer gene specific primer, NH62: 5'-CAATATACCCGAAGATATCGCCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K102661-0022-518-D05C-NH62
CATCTACCTGGTTGTGTTGATGGATGGGTGAACGAGTCAAGGTCGCCGACTCGGTTAATG
ACTCAGTCGTTGAGGATTGGGTCAATACTGGTGGTAGACCGCTCGGAATCGCGTTCGGAA
TCCACGGCGAAGTCATTGTCGCAGACGTACACAAGGTTGGGGTTTTGAAATCGAATTTTG
AAAAATCACAATCTTAGGTTTGACTTTGTTGTTATGTATAGGGACTGTTGAATATAAGCG
GTGACGGAAAGAAGTATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGA
CAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAA
ATCTTTATTTCGACGTGTCTACATTCACGTCCAAAGGGG

Last Updated on 06.12.2018 16:11