Sequences from confirmation amplicon


Confirmation sequences for line 518H10 and hit At2g42230 are shown.
Line ID 518H10
Locus At2g42230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17594478
Primers used for PCR 28uj - 8474, show primer details
   
Sequence Name 30-K117118-0022-518-H10C-28uj
GenBank Accession LN499887 [GenBank]
Sequencing primer gene specific primer, 28uj: 5'-GAAATTAACCCACGAACACAATCT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117118-0022-518-H10C-28uj
CTAACAAAAAGGGGAATCTAACCAAAAAGGTAGTGAACTGGTCAGGGTCCACACAAGAAG
CCGGTTCAGCTTGTTTATCAGAGGCACCAGTCGGATGTGATAGTGAGTCATGCGGATCAA
TTGCTCCGAGCGCCAAGGATTCGTTCCATTTGTTGATAGTTGGCTGAATTCCTACCTCGG
TTATGTGCTCCTCCAAGTGTGAGTAAAATGTATTATATGGAGCAACCTGGAACAGATAAC
AGTCACATTGATGCCAACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTAATTAT
   
Sequence Name 08-K117117-0022-518-H10C-8409
GenBank Accession LN489902 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K117117-0022-518-H10C-8409
ATTTCATTGAATATTCCTGTTGGCATCAATGTGACTGTTATCTGTTCCAGGTTGCTCCAT
ATAATACATTTTACTCACACTTGGAGGAGCACATAACCGAGGTAGGAATTCAGCCAACTA
TCAACAAATGGAACGAATCCTTGGCGCTCGGAGCAATTGATCCGCATGACTCACTATCAC
ATCCGACTGGTGCCTCTGATAAACAAGCTGAACCGGCTTCTTGTGTGGACCCTGACCAGT
TCACTACCTTTTTGGTTAGATTCCCCTTTTTGTTAGTTTAATTTTCCTCTAGTAACTAGT
GATATTGGTTATTAAGATTGTGTTCGTGGGTTAATTTC

Last Updated on 06.12.2018 16:11