Sequences from confirmation amplicon


Confirmation sequences for line 522C07 and hit At3g30720 are shown.
Line ID 522C07
Locus At3g30720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:12348885
Primers used for PCR 18hj - 8474, show primer details
   
Sequence Name 50-K112479-0022-522-C07C-18hj
GenBank Accession LN507217 [GenBank]
Sequencing primer gene specific primer, 18hj: 5'-TCTACATTTCAAACCCATAGGTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K112479-0022-522-C07C-18hj
TATGATTCACACAACAAGAAGATAAGTGTACATGTCTTCTTAGGTGACAATATGAGATAC
AATGATATTCATTTCAAGAATAAAAACGTTTCAAAATATTTTTAAATAATTTCTAAAAAT
CTGCAATTATGTAAACATAATAAATTTGTCAATATATATTTTTTTTTTCATAAAAAATTT
GAAAATGGCATGTAAAAAACATTCTTATGCCTTCATGAAATAGCCAATTAAAATGTTTCA
CTCGAATTGATGTCGTGGCAAAATCTCAGCCTTTCCTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAAATGTCCGCAGCGTTATAAA
   
Sequence Name 20-K112478-0022-522-C07C-8409
GenBank Accession LN495274 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K112478-0022-522-C07C-8409
AAGGCTGAGATTTCGCCCGACATCAATCCGAGTGAAACATTCTAATTGGCTATTTCATGA
AGGCATAAGAATGTTTTTTACATGCCATTTTCAAATTTTTTATGAAAAAAAAAATATATA
TGGACAAATTTATTATGTTTACATAATTGCAGATTTTTAGAAATTATTTAAAAATATTTT
GAAACGTTTTTATTCTTGAAATGAATATCATTGTATCTCATATTGTCACCTAAGAAGACA
TGTACACTTATCTTCTTGTTGTGTGAATCATATTGTCACCTAAAAAGACCTTGCTAGCTT
TGGGACCTATGGGTTTAAAATGTAAA

Last Updated on 06.12.2018 16:11