Sequences from confirmation amplicon


Confirmation sequences for line 522C09 and hit At2g28950 are shown.
Line ID 522C09
Locus At2g28950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:12432367
Primers used for PCR NG47 - 8474, show primer details
   
Sequence Name 52-K102368-0022-522-C09C-8409
GenBank Accession LN507771 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K102368-0022-522-C09C-8409
CGCGGGCATGAGACCCGACATAAATGCTGCACCGGCTCGCGCCAAGAATCAAAAGACAAG
ACTTTTTGACCGTACCCCTATACTTCTAACATATTATGTTTGACACCCTAAATGTTTTAA
TTCTACAACATTAAACCAAATTATTCAGAATCTATAAAAATAAGACTTATCAACTACACC
ATAATATTTATAAATGTTATGTTTGATACTCTAAACTTCTAGTTCTGTCGGCATTAACCC
TAATTGAACAGAATATAGACGCAAGACTTCTCGACTAGTACCCTAAAACTTTTAATCCTG
CCACATTAACCCCAATCATTTGACCCAATACTTCTAAGATGTTAATTTCATACCCCAAAG
TTTAAAATCTATCAAATTAACTCCAATTTGTCTTTCTCAACTAAACAAAGCCTAGGTTTA
AGCTGATATATAAGATTCCACTGAAACAATAAATTACTCTTCTTACCGGCGGAAAGAGAC
GGGGACGATTCCGGCACGATACTCAGCGATCTTGAGAAACATAGGCATAGCAAGATCAAA
GTGAGGTCTAGGAGGGTTAA
   
Sequence Name 52-K102369-0022-522-C09C-NG47
GenBank Accession LN507772 [GenBank]
Sequencing primer gene specific primer, NG47: 5'-TAACCCTCCTAGACCTCACTTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K102369-0022-522-C09C-NG47
GTGCCGGAATCGTCCCCGTCTCTTTCCGCCGGTAAGAAGAGTAATTTATTGTTTCAGTGG
AATCTTATATATCAGCTTAAACCTAGGCTTTGTTTAGTTGAGAAAGACAAATTGGAGTTA
ATTTGATAGATTTTAAACTTTGGGGTATGAAATTAACATCTTAGAAGTATTGGGTCAAAT
GATTGGGGTTAATGTGGCAGGATTAAAAGTTTTAGGGTACTAGTCGAGAAGTCTTGCGTC
TATATTCTGTTCAATTAGGGTTAATGCCGACAGAACTAGAAGTTTAGAGTATCAAACATA
ACATTTATAAATATTATGGTGTAGTTGATAAGTCTTATTTTTATAGATTCTGAATAATTT
GGTTTAATGTTGTAGAATTAAAACATTTAGGGTGTCAAACATAATATGTTAGAAGTATAG
GGGTACGGTCAAAAAGTCTTGTCTTTTGATTCTTGGCGCGAGCCGGTGCAGCATTTATGT
CGGGTCTCATGCCCGCTGAAATCTACATGGATCAGCAATGAGTATGAGGGTCAATA

Last Updated on 06.12.2018 16:11