Sequences from confirmation amplicon


Confirmation sequences for line 522F02 and hit At2g31300 are shown.
Line ID 522F02
Locus At2g31300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:13347858
Primers used for PCR ND04 - 8474, show primer details
   
Sequence Name 20-K032005-022-522-F02C-ND04
GenBank Accession LN495101 [GenBank]
Sequencing primer gene specific primer, ND04: 5'-GTGCAATCAACAGAACCTCTTGTA-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K032005-022-522-F02C-ND04
GCAGCTCTCTGTAAAGCTTTCTCTACCAAAAATATTCTATAAGTTGGCCACCAGATTGAG
TATAGAGTACATGATCCCTAAAAATAGAATATCAATCTCCACAAACTAGTTAATCTTCTA
AATCATTTTTTCTCGTATGCTTTTTTATGTTGCTGTGCTCTCTGATTGTCTGTTATGCAT
TAGGTTCTTCTGGCAACGACATCAACAGATGGAAAATGTCGGGTATTTTCAACATTCATC
AAAGGAGTTGACACAAAGTATGCTCATTACTTGAACGTCTACTTTTAAGACATTTTTTCA
AACATGAGGCCATTTCTTATTTTTCTTTCAATGGGCTGATACAGGGATTCAAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
   
Sequence Name 19-K032005-022-522-F02C-8409
GenBank Accession LN494650 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K032005-022-522-F02C-8409
ATTTTGATCCCTGTATAGCCCATTGAAAGAAAAATAAGAAATGGCCTCATGTTTGAAAAA
ATGTCTTAAAAGTAGACGTTCAAGTAATGAGCATACTTTGTGTCAACTCCTTTGATGAAT
GTTGAAAATACCCGACATTTTCCATCTGTTGATGTCGTTGCCAGAAGAACCTAATGCATA
ACAGACAATCAGAGAGCACAGCAACATAAAAAAGCATACGAGAAAAAATGATTTAGAAGA
TTAACTAGTTTGTGGAGATTGATATTCTATTTTTAGGGATCATGTACTCTATACTCAATC
TGGTGGCCAACTTATAGAATATTTTTGGTAGAGAAAGCTTTTACAGAGAGCCTGCTTAAT
ATTTTGGCTACATGAACTACAAGAGGTTCTGTTGATTGCA
Primers used for PCR 56wp - 8474, show primer details
   
Sequence Name 49-K118546w-0022-522-F02C-56wp
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 56wp: 5'-GCAATTATTGGTAGTGGTTCGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K118546w-0022-522-F02C-56wp
AGAACTGAAAATTTGATAGTATGCAAACCCCTAAGAAAGAGAATTTTAAGCAGATATTTC
TGAGAGTTCACACCATATTCCAGTATCATCTGCAGCAAATACCATCGGGTTGCTGTCATA
TCCAACCCCTATCACCATTTTCTCAGAAATAAACAGGACCTGAAATAGCAATTTCCCCAT
AATATATCAATTCAGGACATAACACACCGGAGAAACCAGGTTCTCAAAAGTAAAATAAAG
GTGGATGCTGACCCACATCACGAAGAGGCAAATCTCGGAATGCAACGCTTTGGGCTAAAG
GAGAAGGGCCAACATCATCAACAAAGTAAATCATGGAGCTATGACCTATACATGATTACA
TAAACACGCAAAGTATGACAGATGGTAGTAAAAACTGCAACCAAGTTGAAGTTCCATTGT
ATTATTTCAGTAGAGCGCAATGGAAAAACATCCCAAGTCTGAAGGATTTTCTGACCTACA
TATGCTAAGGTGTTTCCACTTGGAGACCATTTAACACCAAATGCCCAAGAATATGAGAGA
TCAAGCTGAAGAATTTGCTGTTTCAACAAAGCCACAGTAGAAAAAAAAACTCCCTAACAT
TAGATGAGAGAGTAGACGGAACATATATATGGCACAAGAATTATAATATTTACTAAAAGA
AGGAAAGCCAGCCAAACTAGTCGAACCTCCCCAAATTTCGTTTCCATATTCAATTGTAAA
TGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGA
AAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 29-K118545w-0022-522-F02C-oriL
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K118545w-0022-522-F02C-oriL
ATTTCATTGAATAGGAAACGAAATTTGGGGAGGTTCGACTAGTTTGGCTGGCTTTCCTTC
TTTTAGTAAATATTATAATTCTTGTGCCATATATATGTTCCGTCTACTCTCTCATCTAAT
GTTAGGGAGTTTTTTTTTCTACTGTGGCTTTGTTGAAACAGCAAATTCTTCAGCTTGATC
TCTCATATTCTTGGGCATTTGGTGTTAAATGGTCTCCAAGTGGAAACACCTTAGCATATG
TAGGTCAGAAAATCCTTCAGACTTGGGATGTTTTTCCATTGCGCTCTACTGAAATAATAC
AATGGAACTTCAACTTGGTTGCAGTTTTTACTACCATCTGTCATACTTTGCGTGTTTATG
TAATCATGTATAGGTCATAGCTCCATGATTTACTTTGTTGATGATGTTGGCCCTTCTCCT
TTAGCCCAAAGCGTTGCATTCCGAGATTTGCCTCTTCGTGATGTGGGTCAGCATCCACCT
TTATTTTACTTTTGAGAACCTGGTTTCTCCGGTGTGTTATGTCCTGAATTGATATATTAT
GGGGAAATTGCTATTTCAGGTCCTGTTTATTTCTGAGAAAATGGTGATAGGGGTTGGATA
TGACAGCAACCCGATGGTATTTGCTGCAGATGATACTGGAATATGGTGTGTAACTCTCAG
AAATATCTGCTTAAAATTCTCTTTCTTAGGGGTTTGCATACTATCAAATTTTCAGTTCTT
TTATTCCTCTTAGATCACAGGCCGGATTGTTGGTTCTGAAACGAAC
Primers used for PCR FP93 - 8474, show primer details
   
Sequence Name 48-K118546w-0022-522-F02C-FP93
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, FP93: 5'-CTTGGAGACCATTTAACACCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K118546w-0022-522-F02C-FP93
TTTGCTGTTTCACAAAGCCACANTAGAAAAAAAAACTCCCTAACATTAGATGAGAGAGTA
GACGGAACATATATATGGCACAAGAATTATAATATTTACTAAAAGAAGGAAAGCCAGCCA
AACTAGTCGAACCTCCCCAAATTTCGTTTCCATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTAT
   
Sequence Name 28-K118545w-0022-522-F02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K118545w-0022-522-F02C-8409
CATTGAATATGGAAACGAAATTTGGGGAGGTTCGACTAGTTTGGCTGGCTTTCCTTCTTT
TAGTAAATATTATAATTCTTGTGCCATATATATGTTCCGTCTACTCTCTCATCTAATGTT
AGGGAGTTTTTTTTTCTACTGTGGCTTTGTTGAAACAGCAAATTCTTCAGCTTGATCTCT
CATATTCTTGGGCATTTGGTGTTAAATGGTCTCCAAG

Last Updated on 06.12.2018 16:11