Sequences from confirmation amplicon


Confirmation sequences for line 523D09 and hit At4g18360 are shown.
Line ID 523D09
Locus At4g18360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:10146661
Primers used for PCR TK29 - 8474, show primer details
   
Sequence Name 15-K103674-0022-523-D09C-TK29
GenBank Accession LN492939 [GenBank]
Sequencing primer gene specific primer, TK29: 5'-ATCTATCACAAGCTTGCCAATTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K103674-0022-523-D09C-TK29
GTAAACAATATACTAAGAATGAGAATATGCATCAACCTTTTGCTTGAAGTTTTCATTATG
TTTTAAGATAATTTCTATGATCATTCAAATTTTTGGCAGCAAGAATTGCTGTTGAATATG
GAGCTGCAGGGATAATAGTCTCTAACCACGGAGCTCGTCAGCTAGATTATGTTCCTGCAA
CTATAGTGGCCTTAGAAGAGGTAAACTTTTAAATGGATCAAGATTTAAAACTGTTAAAAC
AAAAAGTTTCAGCTTTCTTCATTGATTAATCAAGCAAACACAAATCGCAGGTGGTTAAAG
CCGTGGAGGGTCGGATTCCGGTTTTTCTTGACGGTGGGGTTCGCCGTGGAACCGATGTCT
TTAAGGCATTGGCTCTTGGCGCTTCAGGCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCA
   
Sequence Name 31-K103675-0022-523-D09C-8409
GenBank Accession LN500130 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103675-0022-523-D09C-8409
GCCATTTACAATTGAATATATCCTGCCTGAAGCGCCAAGAGCCAATGCCTTAAAGACATC
GGTTCCACGGCGAACCCCACCGTCAAGAAAAACCGGAATCCGACCCTCCACGGCTTTAAC
CACCTGCGATTTGTGTTTGCTTGATTAATCAATGAAGAAAGCTGAAACTTTTTGTTTTAA
CAGTTTTAAATCTTGATCCATTTAAAAGTTTACCTCTTCTAAGGCCACTATAGTTGCAGG
AACATAATCTAGCTGACGAGCTCCGTGGTTAGAGACTATTATCCCTGCAGCTCCATATTC
AACAGCAATTCTTGCTGCCAAAAATTTGAATGATCATAGAAATTATCTTAAAACATAATG
AAAACTTCAAGCAAAAGGTTGATGCATATTCTCATTCTTAGTATATTTGTTTTACCATCC
TCAGCTGTAATAACACCCTTGACAAGAATTGGCAAGCTTGTGAT

Last Updated on 06.12.2018 16:11