Sequences from confirmation amplicon


Confirmation sequences for line 523G11 and hit At5g14420 are shown.
Line ID 523G11
Locus At5g14420 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4650498
Primers used for PCR AB16 - 8474, show primer details
   
Sequence Name 57-K027154-022-523-G11C-8409
GenBank Accession LN509299 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K027154-022-523-G11C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCGGACATGTAGATG
CATATTTACATGAAGCCATGAAGCCATCTACATGGTGATGAAGCAGAAGTTGACCTAAAC
GATGACTGTCTCCAGTTTTCTTTAGAATTCCCTGTCCCCATCAAACTTAACAAAAAACTA
AACCTTTTTAGATTCAAATCAAACCTCTCAGGTCAGTTCCGTACCCTGCAAAACAAAGAT
TTAGCCTTTAGAGTATATTCATGAAACCCCAGAAAAAAAAAGCAGAAACTTTGTTTAAGA
TTCTGACATTATTAGAAGATGCAATTTACCTATACCTAATTCGTTAAGAAAAGGTGAAAG
CTTTATCTTTGACAGATGTTGAATTATCTCACCGCTCTTTAAAATCGAAGGTTGGTAGAG
ATGTTTATATAAAGAAAGGAGATTCAATCACGTCACGTAATTTTCCA
   
Sequence Name 61-K027154-022-523-G11C-AB16
GenBank Accession LN510418 [GenBank]
Sequencing primer gene specific primer, AB16: 5'-CGTATTGTTGTGGAAAATTACGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K027154-022-523-G11C-AB16
TGAATCTCCTTTCTTTATATAAACATCTCTACCAACCTTCGATTTTAAAGAGCGGTGAGA
TAATTCAACATCTGTCAAAGATAAAGCTTTCACCTTTTCTTAACGAATTAGGTATAGGTA
AATTGCATCTTCTAATAATGTCAGAATCTTAAACAAAGTTTCTGCTTTTTTTTTCTGGGG
TTTCATGAATATACTCTAAAGGCTAAATCTTTGTTTTGCAGGGTACGGAACTGACCTGAG
AGGTTTGATTTGAATCTAAAAAGGTTTAGTTTTTTGTTAAGTTTGATGGGGACAGGGAAT
TCTAAAGAAAACTGGAGACAGTCATCGTTTAGGTCAACTTCTGCTTCATCACCATGTAGA
TGGCTTCATGGCTTCATGTAAATATGCATCTACATGTCCGGATATATTCAATTGTAAATG
GCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
ATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGA
AAGCAATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11