Sequences from confirmation amplicon


Confirmation sequences for line 526B04 and hit At3g03120 are shown.
Line ID 526B04
Locus At3g03120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:717353
Primers used for PCR JU35 - 8474, show primer details
   
Sequence Name 12-K023836-022-526-B04C-8409
GenBank Accession LN491392 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K023836-022-526-B04C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGATCCATCTCTCACCCCAAAAAAGCTT
AAACCCCCACAGACGAATTTTACTTTCTCTTATCGATAAACCAAAACAGAAATTGAAAGT
GAAGAAACACAGAGAGAATCAAAGCTAAGATGATGATGGTCGATTTCTCCAGGAATTGCA
GAGAGTTTCCCAAGAAAGCAAAGGTTGCGTTTTCGGTACAATGGATTCCTTATAANNANG
GNNNAAAAAATGATACNGGTTTTGGTTGGAGTTGAAAGAAAGTTAGTCCAAGGAAGTGTA
GAACTAGTGAGAAACGATTATCTGCTTGGTCCTCCTGTTAGTGAGATCAANNNTNNNGGA
TAAACCAATAATGCACAAAAGGGCAAACCAATTATGCTATGAGAA
   
Sequence Name 16-K023836-022-526-B04C-JU35
GenBank Accession LN493259 [GenBank]
Sequencing primer gene specific primer, JU35: 5'-ATAAGGAATCCATTGTACCGAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K023836-022-526-B04C-JU35
ACCTTTGCTTTCTTGGGAAACTCTCTGCAATTCCTGGAGAAATCGACCATCATCATCTTA
GCTTTGATTCTCTCTGTGTTTCTTCACTTTCAATTTCTGTTTTGGTTTATCGATAAGAGA
AAGTAAAATTCGTCTGTGGGGGTTTAAGCTTTTTTGGGGTGAGAGATGGATCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTT
ATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGG
CGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA
CGT
Primers used for PCR JU34 - 8474, show primer details
   
Sequence Name 25-K027353-022-526-B04C-8409
GenBank Accession LN497293 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K027353-022-526-B04C-8409
CATTTACATTGAATATATCCGGACATGTAGATTTCAAAACAATGAACGCTAGTATAACGA
TTAACGATGGGTAACACGCGATTCCGTCCAGTAAACGATCAAATACAGAAATCAAATACA
GAAGATGGAGAGAAGGAGAGTACCCTCATCTCCTGGTTGCCGAAGAAGGTATCGAAAAGC
TTGCGAAAAGTTTGACTCGGATTTTGAAATAATATGAAAAAGTGTTTGATTTGTGCTAAT
CATTGATGTATCCTGGGATTTATTATATGTTCAAAGAGTTACTTTTTAGGTCCGGTATAT
ATGCTTGTAATCTAAACTCTTGCTAGTACTTTTAGGTACCCTGAAGATTCAAGTTCCCTT
CTACTATAGTATAGTACCTACACTGTTTAGAATCTTGATGTAGCCAATCCACTTTCATAG
ACAGTTGGGTTACTTGTGTATCCATGAAATCATTCACACAAGTTAGCTAGTCCAGCTTTT
GTTTTGTGTATTGACCCAGCTTATTAGCTATCAAGTGCAATAACTCTAGATATTAAGTGC
TGTTACAGATTATGTGGTGTTCTTGTTGATATATTACTAATCTATGTGGTGTGGTTTCTC
ACTGT
   
Sequence Name 26-K027353-022-526-B04C-JU34
GenBank Accession LN497767 [GenBank]
Sequencing primer gene specific primer, JU34: 5'-CATCATATCATGCACAGTGAGAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K027353-022-526-B04C-ju34
TCACAAGAACCCACATAATCTGTAACAGCACTTAATATCTAGAGTTATTGCACTTGATAG
CTAATAAGCTGGGTCAATACACAAAACAAAAGCTGGACTAGCTAACTTGTGTGAATGATT
TCATGGATACACAAGTAACCCAACTGTCTATGAAAGTGGATTGGCTACATCAAGATTCTA
AACAGTGTAGGTACTATACTATAGTAGAAGGGAACTTGAATCTTCAGGGTACCTAAAAGT
ACTAGCAAGAGTTTAGATTACAAGCATATATACCGGACCTAAAAAGTAACTCTTTGAACA
TATAATAAATCCCAGGATACATCAATGATTAGCACAAATCAAACACTTTTTCATATTATT
TCAAAATCCGAGTCAAACTTTTCGCAAGCTTTTCGATACCTTCTTCGGCAACCAGGAGAT
GAGGGTACTCTCCTTCTCTCCATCTTCTGTATTTGATTTCTGTATTTGATCGTTTACTGG
ACGGAATCGCGTGTTACCCATCGTTAATCGTTATACTCGCTTTCATTGTTTTGAAATCTA
CATGTCCGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACA
AATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAAT
CTTTATTTCGACGTGTCTAC
Primers used for PCR 07lq - 8474, show primer details
   
Sequence Name 24-K118781-0022-526-B04C-8409
GenBank Accession LN497203 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K118781-0022-526-B04C-8409
TTGATCCATCTCTCACCCCAAAAAAGCTTAAACCCCCACAGACGAATTTTACTTTCTCTT
ATCGATAAACCAAAACAGAAATTGAAAGTGAAGAAACACAGAGAGAATCAAAGCTAAGAT
GATGATGGTCGATTTCTCCAGGAATTGCAGAGAGTTTCCCAAGAAAGCAAAGGTTGCGTT
TTCGGTACAATGGATTCCTTATGTACAGTATAGAGAGATTCCACTTGGTCTTACATTTTT
ATTTCGGTTTACAAGTCTTTGAAATTAAGACCGGTTTATAACCGGTTTGAGGTTCGATTA
ATTTTGAGATCCTAAACATTTTTCATTTAATAGTAACAAATCCAACAAAAAAGATTGTAA
TTTAGAAAACCTAACACTTGTCTTTTCTAAGCTTTCCATTTCTCAAGATCTCTACAAAAA
AAATTCCATTTCTCTTTTCGAGAGCCTTCTTCTTATAAAATATAAAAACAAGACTTAAGA
ATCGGCCATGTCGTCTTGAATTTCGCTGGGAGCTATAAGTCCAGGAATCGAAAGCATTCT
TTGTCTCTCTCTCTCCATTTGGGCAGCAGCTTCCTCAGCATATAAATCTTTGTTATCCTG
AACAAAAATAATCCATTCATTAGAGTTTTTGATGAAATGTGGTATAAAAGAAGATACCAA
TCTAATCTGTAGCAATTACTTATATACCTGAGCAGAANATTCCTTGGACTGTATAAGGAA
GTCGCGTATGTTATCCTTAAATCTGCCAACATCGTTC
   
Sequence Name 64-K118782-0022-526-B04C-07lq
GenBank Accession LN511301 [GenBank]
Sequencing primer gene specific primer, 07lq: 5'-ACTTTACGAGTCGAGAAACGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K118782-0022-526-B04C-07lq
ACGCGACTTCTTATACGTCCAAGGAATTTTCTGCTCAGGTATATAAGTAATTGCTACAGA
TTAGATTGGTATCTTCTTTTATACCACATTTCATCAAAAACTCTAATGAATGGATTATTT
TTGTTCAGGATAACAAAGATTTATATGCTGAGGAAGCTGCTGCCCAAATGGAGAGAGAGA
GACAAAGAATGCTTTCGATTCCTGGACTTATAGCTCCCAGCGAAATTCAAGACGACATGG
CCGATTCTTAAGTCTTGTTTTTATATTTTATAAGAAGAAGGCTCTCGAAAAGAGAAATGG
AATTTTTTTTGTAGAGATCTTGAGAAATGGAAAGCTTAGAAAAGACAAGTGTTAGGTTTT
CTAAATTACAATCTTTTTTGTTGGATTTGTTACTATTAAATGAAAAATGTTTAGGATCTC
AAAATTAATCGAACCTCAAACCGGTTATAAACCGGTCTTAATTTCAAAGACTTGTAAACC
GAAATAAAAATGTAAGACCAAGTGGAATCTCTCTATACTGTACATAAGGAATCCATTGTA
CCGAAAACGCAACCTTTGCTTTCTTGGGAAACTCTCTGCAATTCCTGGAGAAATCGACCA
TCATCATCTTAGCTTTGATTCTCTCTGTGTTTCTTCACTTTCAATTTCTGTTTTGGTTTA
TCGATAAGAGAAAGTAAAATTCGTCTGTGGGGGTTTAAGCTTTTTTGGGGTGAGAGATGG
ATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCATTCAAAAATGT

Last Updated on 06.12.2018 16:11