Sequences from confirmation amplicon


Confirmation sequences for line 526G03 and hit At2g11270 are shown.
Line ID 526G03
Locus At2g11270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:4484657
Primers used for PCR 33td - 8474, show primer details
   
Sequence Name 17-K116610-0022-526-G03C-33td
GenBank Accession LN494019 [GenBank]
Sequencing primer gene specific primer, 33td: 5'-TCACATACAATTTGATCTCCAAGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K116610-0022-526-G03C-33td
GGGATTAATCTCATGATTAATTTTTTTAAGCATTATCAAATCCCAAATCACAAGTCTTTT
CTTAACAAATTAAATGTGTTAGTTCTTTAAAAATGAGATGATGAGTATTTTTAAGAGTGA
ATTGGTGTAATAGCCCTATTGTTGTTTAAGTTAAAGGGAATGATCAAATGTCTGAATACC
ATTTCCTGAAGTATATGGACGAAACGCAGAAACAGAACATTGACCCATCTTTTTTGAGAG
AGAGATCAGCAATGAGTATGATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCACGATTATTAT
   
Sequence Name 01-K116609-0022-526-G03C-8409
GenBank Accession LN486739 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K116609-0022-526-G03C-8409
CTCATTGCTGATCTCTCTCTCAAAAAAGATGGGTCAATGTTCTCTTTCTGCGTTTCGTCC
ATATACTTCAGGAAATGGTATTCAGACATTTGATCATTCCCTTTAACTTAAACAACAATA
GGGCTATTACACCAATTCACTCTTAAAAATACTCATCATCTCATTTTTAAAGAACTAACA
CATTTAATTTGTTAAGAAAAGACTTGTGATTTGGGATTTGATAATGCTTAAAAAAATTAA
TCATGAGATTATATCCCTTAATCTCTCGTCTATCCTTGATGGATCAATCTGATTGCTTGG
AGATCAAATTGTATGTGA

Last Updated on 06.12.2018 16:11