Sequences from confirmation amplicon


Confirmation sequences for line 526H07 and hit At3g52920 are shown.
Line ID 526H07
Locus At3g52920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19625340
Primers used for PCR 12vk - 8474, show primer details
   
Sequence Name 59-K117876-0022-526-H07C-12vk
GenBank Accession LN510096 [GenBank]
Sequencing primer gene specific primer, 12vk: 5'-GATTTGTTCTCATTGTTCAGCAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K117876-0022-526-H07C-12vk
AGCTGAACTTTCCCACGATTCTTCTCGTTGAACGTATCCAATGCTTCTTTGTATTCCCTT
TCCTGTTGTCATATCATCACATAAAACAAAAACATGAGTTTCAGGTTTTGTTCATTGAAA
AACAAATCTTTAAAGTTGACTAATGCGATATCAAATCTTTTAGTCAACACCATAACAACC
ACAAATCGATTCAGTTGACATTTAGGTTTGAGTTTTGGGTTTGGTAAAGAGCAAAACTTT
TCATAGCAACAGGTAGTGAGAATGAAGCAACTGCTCTTTGACAAGTGTACTTACCTTCTT
TTGAACAGTAGAGCCTAATGGTTTGAGTTCTTTGTTGACGCTATCAATCTTCTTACGCAC
CCAATTAACTTCCTTCCTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 35-K117875-0022-526-H07C-8409
GenBank Accession LN501991 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K117875-0022-526-H07C-8409
TTTACATTGAAATATCCTGAGGAAGGAGTTAATTGGGTGCGTAAGAAGATTGATAGCGTC
AACAAAGAACTCAAACCATTAGGCTCTACTGTTCAAAAGAAGGTAAGTACACTTGTCAAA
GAGCAGTTGCTTCATTCTCACTACCTGTTGCTATGAAAAGTTTTGCTCTTTACCAAACCC
AAAACTCAAACCTAAATGTCAACTGAATCGATTTGTGGTTGTTATGGTGTTGACTAAAAG
ATTTGATATCGCATTAGTCAACTTTAAAGATTTGTTTTTCAATGAACAAAACCTGAAACT
CATGTTTTTGTTTTATGTGATGATATGACAACAGGAAAGGGAATACAAAGAAGCATTGGA
TACGTTCAACGAGAAGAATCGTGAGAAAGTTCAGCTGATCACCAAGCTAATGGAGGTTAG
TTTCGAGTTGTTGGTTATAGTTTTGCTGAA

Last Updated on 06.12.2018 16:11