Sequences from confirmation amplicon


Confirmation sequences for line 527A02 and hit At4g29780 are shown.
Line ID 527A02
Locus At4g29780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14581227
Primers used for PCR 34kh - 8474, show primer details
   
Sequence Name 78-K113549-0022-527-A02C-34kh
GenBank Accession LN513717 [GenBank]
Sequencing primer gene specific primer, 34kh: 5'-CACTTGTACTTCTGTCTGAGCCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K113549-0022-527-A02C-34kh
TCTTTTTCAGTCGACCAATTTTGTGAAAAATCGAAAGAGAAGAAGCTTATACTTAAGAGG
TTTCAGATTCAATTTGTATAGAAAATACATGAACTAAAGATTACACAATGCTAATAAGTA
ACACAAACAATATCATCAGCCACTTCTACTCTCAATTCAACTATTCTTTCTGTATCCAAC
CAATCCCTAAAATGTTTTTATTCCTAATTTGGAAAAAGAATTTGCAATAAAAAATCAGTT
TCAAAATAAGAAAAGGTGAAAAACAGAGCCTATAGAGTTCTTGTCCCGGCAAGTCCACGA
TGCAAGAGATTGTGAGAGATATGATCCCTCGTATTAACCGCACTAGCAGATCGGATATTA
TTCTCCGGCACCGCCACATCATCAAAAACCTCAAACTTCAACTCCGGCAACATCTCCTCC
TTCCTCATCTCACAAATGTTATGCAACACACAACAAGCTCCAAGCACGTACGGCAGATCC
TGAAGCTTCACCTCCGTCCGTTTCTGCAAACAAGCCCACCGTCCGGATATATTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 13-K113548w-0022-527-A02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K113548w-0022-527-A02C-8409
TCGGACTGAGCCTTTCATTGAATATATCCGGACGGTGGGCTTGTTTGCAGAAACGGACGG
AGGTGAAGCTTCAGGATCTGCCGTACGTGCTTGGAGCTTGTTGTGTGTTGCATAACATTT
GTGAGATGAGGAAGGAGGAGATGTTGCCGGAGTTGAAGTTTGAGGTTTTTGATGATGTGG
CGGTGCCGGAGAATAATATCCGATCTGCTAGTGCGGTTAATACGAGGGATCATATCTCTC
ACAATCTCTTGCATCGTGGACTTGCCGGGACAAGAACTCTATAGGCTCTGTTTTTCACCT
TTTCTTATTTTGAAACTGATTTTTTATTGCAAATTCTTTTTCCAAATTAGGAATAAAAAC
ATTTTAGGGATTGGTTGGATACAGAAAGAATAGTTGAATTGAGAGTAGAAGTGGCTGATG
ATATTGTTTGTGTTACTTATTAGCATTGTGTAATCTTTAGTTCATGTATTTTCTATACAA
ATTGAATCTGAAACCTCTTAAGTATAAGCTTCTTCTCTTTCGATTTTTCACAAAATTGGT
CGACTAGAAAAAGAAGAAGCAATTCCACTTTTATTACAAGCTCAGGCTCAGACAGAAGTA
CAAGTG

Last Updated on 06.12.2018 16:11