Sequences from confirmation amplicon


Confirmation sequences for line 527D03 and hit At3g16950 are shown.
Line ID 527D03
Locus At3g16950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5786913
Primers used for PCR 50gp - 8474, show primer details
   
Sequence Name 44-K112250-0022-527-D03C-50gp
GenBank Accession LN505229 [GenBank]
Sequencing primer gene specific primer, 50gp: 5'-AAGGAGAAGGAATAGCTAAGGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K112250-0022-527-D03C-50gp
AGAGATCTCATTGCTTTTCATTATGCAGATGATATACCGACCTGACAATGGTGAAATCTT
AGGAGTTCATATATTTGGACTGCATGCAGCTGACCTTATCCATGAAGCTTCTAATGCGAT
TGCTCTAGGAACGCGTATTCAGGTAGGATTTCATTGATTAGTGATTACTATTGAATCTAG
CTCGTAGCCTTTGATATTGATAATATCCTACAGCGAAATCAAGTCATATACATAGTTCCT
GCTGAAACTACTAAACGCATTATTTGTATCTGTTTCTTGATCCCCTGCATGTTTTAATGG
TATCTCTGCAGGACATAAAATTGGCAGTTCATGCACATCCAACACTCTCTGAGGTCCTCG
ACGAACTGTTCAAAGCAGCCAAGGTAAAGCAAAACAGTCTCCTCATGTAAATGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 34-K112249-0022-527-D03C-8409
GenBank Accession LN501467 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K112249-0022-527-D03C-8409
ATGAGGAGACTGTTTTGCTTTACCTTGGCTGCTTTGAACAGTTCGTCGAGGACCTCAGAG
AGTGTTGGATGTGCATGAACTGCCAATTTTATGTCCTGCAGAGATACCATTAAAACATGC
AGGGGATCAAGAAACAGATACAAATAATGCGTTTAGTAGTTTCAGCAGGAACTATGTATA
TGACTTGATTTCGCTGTAGGATATTATCAATATCAAAGGCTACGAGCTAGATTCAATAGT
AATCACTAATCAATGAAATCCTACCTGAATACGCGTTCCTAGAGCAATCGCATTAGAAGC
TTCATGGATAAGGTCAGCTGCATGCAGTCCAAATATATGAACTCCTAAGATTTCACCATT
GTCAGGTCGGTATATCATCTGCATAATGAAAAGCAATGAGATCTCTATGTTCTTTCATGA
GACATAAATAAATCAACAACTTTTGCTTCTCCCACCTTAGCT

Last Updated on 06.12.2018 16:11