Sequences from confirmation amplicon


Confirmation sequences for line 527G10 and hit At2g44100 are shown.
Line ID 527G10
Locus At2g44100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:18242523
Primers used for PCR KF21 - 8474, show primer details
   
Sequence Name 28-K103231-0022-527-G10C-8409
GenBank Accession LN498800 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K103231-0022-527-G10C-8409
ATATGAATTACAACTGTTCTCTGTCATGTGGCGCACCAGTTTATGATGGCTAACGGAAAG
CTTGTTCGTGTCCTTATTCATACGGATGTGACCAAGTACTTGTCTTTTAAAGCTGTTGAT
GGAAGCTATGTTTTTGTCCAAGGCAAGGTACATTGATTACTCATTTTCATTTTTATCTTG
TGCCTCATTCTCTTTTTTTTCCATAGATTTCAAGTCTGTTTGTGCATCTTTTTTTCTCCC
ATGTTTGATCTTGGTCAAAAGCTTTTCACTGATCATTCTAGCTTTGCAGCTTAAGTAATT
TTTTTCTAGTTATATTAACTGTTTATATGCTACAGGTCCAAAAAGTGCCAGCAACTCCCA
TGGAGGCCCTCAAATCTCCTCTTATGGGTATTTTTGAGAAACGTCGAGCTGGAAAGTTTT
TCAGCTATGTTCAAGAATACAACGAGAAGGACCCAAAAACACATGATGGAATGGATTTGA
GGAGAGTTACAACTAAGGATTTGATTGCGTAAGCNGATGTTCTCTTGTCTTTTGCATTAG
GGTG
   
Sequence Name 76-K103232-0022-527-G10C-KF21
GenBank Accession LN513381 [GenBank]
Sequencing primer gene specific primer, KF21: 5'-CACCATAATGCAAAAGACAAGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K103232-0022-527-G10C-KF21
ATCCTTAGTTGTACTCTCCTCAATCCATTCCTCATGTGTTTTTGGGTCCTTCTCGTCGTA
TTCTTGAACATAGCTGAAAAACTTTCCAGCTCGACGTTTCTCAAAAATACCCATAAGAGG
AGATTTGAGGGCCTCCATGGGAGTTGCTGGCACTTTTTGGACCTGTAGCATATAAACAGT
TAATATAACTAGAAAAAAATTACTTAAGCTGCAAAGCTAGAATGATCAGTGAAAAGCTTT
TGACCAAGATCAAACATGGGAGAAAAAAAGATGCACAAACAGACTTGAAATCTATGGAAA
AAAAAGAGAATGAGGCACAAGATAAAAATGAAAATGAGTAATCAATGTACCTTGCCTTGG
ACAAAAACATAGCTTCCATCAACAGCTTTAAAAGACAAGTACTTGGTCACATCCGTATGA
ATAAGGACACGAACAAGCTTTCCGTTAGCCATCATAAACTGGTGCGCCACATTGACNGAG
AACAGTTAGTAATTCATATTGGTAACAAGGAAGCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
NTCCGCAGCGTTNTTATAAAATGAAAGTACATTTTGATAAAACGACNAATTACGATCCGT
CGTATTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACG
TGTCTA

Last Updated on 06.12.2018 16:11