Sequences from confirmation amplicon


Confirmation sequences for line 528F07 and hit At3g08870 are shown.
Line ID 528F07
Locus At3g08870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2702320
Primers used for PCR EQ90 - 8474, show primer details
   
Sequence Name 47-K103248-0022-528-F07C-EQ90
GenBank Accession LN506171 [GenBank]
Sequencing primer gene specific primer, EQ90: 5'-TTTCACTTTCCATAATGGGCTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K103248-0022-528-F07C-EQ90
AGCGGTCTTCATCATTTTGTCCACTATTTTGTTTTCTTTTGGTCGAAGATATTGTCAAGT
ATTAATTATGCAATTACGTGAAATCATGAGAGTCATCTCCTCCACACATATAGCCCAAAA
AGACCTTGACTTTGAACATTTCGACTCTTTCGTCGTTGATAGCATAAGAACATGTCCATA
TTTGAAACTTAAACTCAATGCTCTCTCTTACTAGTTACTTCATACAATCAAAGTCAAAAA
GCTCAAGATACCACTACTCAATGGGCATAGCAAGATCCATAAACTCGTTCATGTTCTTCT
TCTTTCTAATGATATTATCAAACGCTTCTAAAAGCTCTGTTCTTGCTGAGGCCACGACAG
CGAAATTCACATTCATAGGTTTCAAAGAAAACCAAACGGATATTCAAACAGAAGGAGCTT
CCACAATCCAACACGACNATGATCTTCTTAGACTAACTAATCGGAAACAAAACGTCACTG
GAACAGCGTTTTACCGCANTGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAAT
GTAG
   
Sequence Name 43-K103249-0022-528-F07C-8409
GenBank Accession LN504802 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K103249-0022-528-F07C-8409
GCCTTGCGGTAAAACGCTGTTCCGTGACGTTTTGTTTCCGATTAGTTAGTCTAAGAAGAT
CATTGTCGTGTTGGATTGTGGAAGCTCCTTCTGTTTGAATATCCGTTTGGTTTTCTTTGA
AACCTATGAATGTGAATTTCGCTGTCGTGGCCTCAGCAAGAACAGAGCTTTTAGAAGCGT
TTGATAATATCATTAGAAAGAAGAAGAACATGAACGAGTTTATGGATCTTGCTATGCCCA
TTGAGTAGTGGTATCTTGAGCTTTTTGACTTTGATTGTATGAAGTAACTAGTAAGAGAGA
GCATTGAGTTTAAGTTTCAAATATGGACATGTTCTTATGCTATCAACGACGAANGAGTCG
AAATGTTCAAAGTCAAGGTCTTTTTGGGCTATATGTGTGGAGGAGATGACTCTCATGATT
TCACGTAATTGCATAATTAATACTTGACAATATCTTCGACCAAAAGAAAACAAAATAGTG
GACAAAATGATGAAGACCGCTNTGATTTAGCCTAAACATGAGTCTTATTGGTCATAAATT
TTGTTAGCCCATTATGGANAGTGAA
Primers used for PCR 57wp - 8474, show primer details
   
Sequence Name 21-K118742-0022-528-F07C-57wp
GenBank Accession LN495873 [GenBank]
Sequencing primer gene specific primer, 57wp: 5'-ATTGGACCCGAAGAAGATAGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K118742-0022-528-F07C-57wp
GATATCTCACTCTCTTTGAATTTCTTCGTTGCTAAGTAGAGATCTCTGTATCTAAACCTA
TGAGGATAATCAATCTCCCAATCCTCTAGAGTATCTTCTTCTTGGATTCGCCTTTTGTAC
ATCACAAATATAAATAGTAACACAAGCAAGACCAGTGTGACGATCGATAAAGCAACAATC
AAAACAATGACTTGAGAATTGTAACCTTTCTTGTTACTCAAGCGAGGTGGAGGAGGAAGC
CGCGAGATCTCGAGCCAATCCGCCATTGGATTTTCTCCGCAGCTTGAAAAACTCCAACCC
ATTACATAGTGAGCACTCGACTGGTCTTTTCCAGTCGCGGCGGTGAATCCGACGTACATT
TCATCCTTCACAATCTCCGACAGCTCTGAAACTCGTCGCGAGATTAGAGGCTTCTTGGGT
TTAAACTCTAATCTCGTCGGATAGATTGTAACGTTTAAGGTTTCGCTGGATCCGTCGTAA
TCGATAAGAACTCGGATTGGTTCGCCGCTCTCGAGCTGAAAATCTTCCTTTCGATCCTCT
GTGTCGTAGTAAATGAGTGGTTCTTGTACATTGGAGGAGAGGTTGTTGAAATTAAGACCG
ATGTGGTTTCCTCTTCTGTCTGCGCCGTCTTTAAATCCTTGTACCGTGTCGAATTCCACT
GCGAATACGTGATTCGATGGATTACCGTTGTTGGTTCTGTTCAACAGACCCAAATACTGT
GCAGATTCGGCTCCTGGACGATTCGGGGTCGGAGAAAGTGTGAACGTGAAACCAAAACCA
CCGTTGCCGGGACTTGAAGGGAGTATGACGAAGACGAAAGAAGTGCTAAAGGAACAAACT
TTGATATCGGAGGAATTGGTTATTGTAAATATGATCCCCATGTAGAANAAAAATTGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTATTT
   
Sequence Name 05-K118741-0022-528-F07C-oriL
GenBank Accession LN488566 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118741-0022-528-F07C-oriL
TTTACATTACCAATTCCTCCGATATCAAAGTTTGTTCCTTTAGCACTTCTTTCGTCTTCG
TCATACTCCCTTCAAGTCCCGGCAACGGTGGTTTTGGTTTCACGTTCACACTTTCTCCGA
CCCCGAATCGTCCAGGAGCCGAATCTGCACAGTATTTGGGTCTGTTGAACAGAACCAACA
ACGGTAATCCATCGAATCACGTATTCGCAGTGGAATTCGACACGGTACAAGGATTTAAAG
ACGGCGCAGACAGAAGAGGAAACCACATCGGTCTTAATTTCAACAACCTCTCCTCCAATG
TACAAGAACCACTCATTTACTACGACACAGAGGATCGAAAGGAAGATTTTCAGCTCGAGA
GCGGCGAACCAATCCGAGTTCTTATCGATTACGACGGATCCAGCGAAACCTTAAACGTTA
CAATCTATCCGACGAGATTAGAGTTTAAACCCAAGAAGCCTCTAATCTCGCGACGAGTTT
CAGAGCTGTCGGAGATTGTGAAGGATGAAATGTACGTCGGATTCACCGCCGCGACTGGAA
AAGACCAGTCGAGTGCTCACTATGTAATGGGTTGGAGTTTTTCAAGCTGCGGAGAAAATC
CAATGGCGGATTGGCTCGAGATCTCGCGGCTTCCTCCTCCACCTCGCTTGAGTAACAAGA
AAGGTTACAATTCTCAAGTCATTGTTTTGATTGTTGCTTTATCGATCGTCACACTGGTCT
TGCTTGTGTTACTATTTATATTTGTGATGTACAAAAGGCGAATCCAAGAAGAAGATACTC
TAGAGGATTGGGAGATTGATTATCCTCATAGGTTTAGATACAGAGATCTCTACTTAGCAA
CGAAGAAATTTCAAGAGAGTGAGATTATCGGTACTGGAGGATTTGGAATCGTTTA
Primers used for PCR KR76 - 8474, show primer details
   
Sequence Name 20-K118742-0022-528-F07C-KR76
GenBank Accession LN495429 [GenBank]
Sequencing primer gene specific primer, KR76: 5'-GGTTGTTGAAATTAAGACCGATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K118742-0022-528-F07C-KR76
TCGAATTCCCTGCGAATACGTGATTCGATGGATTACCGTTGTTGGTTCTGTTCAACAGAC
CCAAATACTGTGCAGATTCGGCTCCTGGACGATTCGGGGTCGGAGAAAGTGTGAACGTGA
AACCAAAACCACCGTTGCCGGGACTTGAAGGGAGTATGACGAAGACGAAAGAAGTGCTAA
AGGAACAAACTTTGATATCGGAGGAATTGGTTAATTGTAAATATGATCCCCATGTAGAAA
AAAAATTGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTATAAT
   
Sequence Name 04-K118741-0022-528-F07C-8409
GenBank Accession LN488123 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K118741-0022-528-F07C-8409
TATTTACAATTAACCAATTCCTCCGATATCAAAGTTTGTTCCTTTAGCACTTCTTTCGTC
TTCGTCATACTCCCTTCAAGTCCCGGCAACGGTGGTTTTGGTTTCACGTTCACACTTTCT
CCGACCCCGAATCGTCCAGGAGCCGAATCTGCACAGTATTTGGGTCTGTTGAACAGAACC
AACAACGGTAATCCATCGAATCACGTATTCGCAGTGGAATTCGACACGGTACAAGGATTT
AAAGACGGCGCAGACAGAAGAGGAAACCACATCGGTCTTAATTTCAACAAC

Last Updated on 06.12.2018 16:11