Sequences from confirmation amplicon


Confirmation sequences for line 529B08 and hit At4g14350 are shown.
Line ID 529B08
Locus At4g14350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8258531
Primers used for PCR BK04 - 8474, show primer details
   
Sequence Name 76-K104542-0022-529-B08C-BK04
GenBank Accession LN513397 [GenBank]
Sequencing primer gene specific primer, BK04: 5'-CCAAGAGACCACCTAAATCTCAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K104542-0022-529-B08C-BK04
TTCCCAAGAAATCAAGACCACAAATAGGAATAGGTTGGCTTCACCTACCAATCACATTCC
ATTCCATATCCTTTTTTCAACAGAACTTCTGGGGCAATATAGTCAGGAGTGCCAACTGTG
GAATAAGCCTGAAAGTAATGACCTTAACCTAGTAAGTCATATCTAACATAGATGTCTATA
TCAATGGAGAGAATTTGCTTTCAATAACGAAATATACTGGATACTGTGGACTACTCCACA
TCTCTGGAAGAAAAGATGAGGAATAAGTGAAACTTACAAGCATCCTTCTATTTCTACATG
GAAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCACGGTTATT
   
Sequence Name 92-K104543-0022-529-B08C-8409
GenBank Accession LN515194 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>92-K104543-0022-529-B08C-8409
CCATTTACAATTGAATATATTTCCATGTAGAAATAGAAGGATGCTTGTAAGTTTCACTTA
TTCCTCATCTTTTCTTCCAGAGATGTGGAGTAGTCCACAGTATCCAGTATATTTCGTTAT
TGAAAGCAAATTCTCTCCATTGATATAGACATCTATGTTAGATATGACTTACTAGGTTAA
GGTCATTACTTTCAGGCTTATTCCACAGTTGGCACTCCTGACTATATTGCCCCAGAAGTT
CTGTTGAAAAAAGGATATGGAATGGAATGTGATTGGTAGGTGAAGCCAACCTATTCCTAT
TTGTGGTCTTTGATTTCTTTGGTGTAAATAAATAATATGGGTGAATAATCTTGAGATTTA
GGTGGTCTCTTGG

Last Updated on 06.12.2018 16:11