Sequences from confirmation amplicon


Confirmation sequences for line 529C06 and hit At5g40010 are shown.
Line ID 529C06
Locus At5g40010 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16020492
Primers used for PCR CD76 - 8474, show primer details
   
Sequence Name 07-K106611-0022-529-C06C-8409
GenBank Accession LN489286 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106611-0022-529-C06C-8409
ATTTACAATTGATCTCATCAAACAACTCATTATCATCTTCTTCCTTAGCATCCAAGTAAT
TGTTTGCCAACACTTTAAACGCTTCAAAACCGCAGTAGGACATCTCTATATGCTTATCCA
TTCTTCCTTTTCGAATCAAAGCCGGATCTAACTTATCTATGAAATTAGTAGTGAACACAA
TGATCCTCTCACCACCACAAGCTGACCATAACCCATCAATGAAATTCAGTAACCCTGATA
ATGTCACTTTACTTCCTTTATTCTCACCTTGATCTTTCTTCATCTGCTTCTCAATCGGGC
TAGTCTCATCTTCGTCTTCCTCCTCGTCCTTCTTCTGCTTTCTCTGACCAGTTAGGTCAA
GAGAACAATCGATATCTTCAATAACAATTATCGATTTCCCTGAAGTCTCAATCAACAACC
TTCTCAGCTCTGTGTTATCCTTG
   
Sequence Name 23-K106612-0022-529-C06C-CD76
GenBank Accession LN496570 [GenBank]
Sequencing primer gene specific primer, CD76: 5'-TCAAGGATAACACAGAGCTGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106612-0022-529-C06C-CD76
GAATCGATAATTGTTATTGAAGATATCGATTGTTCTCTTGACCTAACTGGTCAGAGAAAG
CAGAAGAAGGACGAGGAGGAAGACGAAGATGAGACTAGCCCGATTGAGAAGCAGATGAAG
AAAGATCAAGGTGAGAATAAAGGAAGTAAAGTGACATTATCAGGGTTACTGAATTTCATT
GATGGGTTATGGTCAGCTTGTGGTGGTGAGAGGATCATTGTGTTCACTACTAATTTCATA
GATAAGTTAGATCCGGCTTTGATTCGAAAAGGAAGAATGGATAAGCATATAGAGATGTCC
TACTGCGGTTTTGAAGCGTTTAAAGTGTTGGCAAACAATTACTTGGATGCTAAGGAAGAA
GATGATAATGAGTTGTTTGATGAGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCAACACGGTTATT

Last Updated on 06.12.2018 16:11