Sequences from confirmation amplicon


Confirmation sequences for line 529D11 and hit At1g32350 are shown.
Line ID 529D11
Locus At1g32350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11667928
Primers used for PCR JL72 - 8474, show primer details
   
Sequence Name 28-K101383-0022-529-D11C-8409
GenBank Accession LN498772 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K101383-0022-529-D11C-8409
CCATTTACAATTGAATATATCCTGTATATATTCGAGCAAAGCTTTGATCCATCCCCCGCT
ATGTTCGAACCTCCGGAGAGATTTCAAGTGCAAAAGCATCCCCCCGACCATTCCCGGCAC
CGCAGCCACCGTCTCTAGCAACATCGCATGGCACATGTGCTTCCTCTATACTCAAAAATC
AAATTATTCAATTTTTGAAAAAACAAAATCATGTTAAAAATAAAAGAACAAATGAAGAAG
ATTTAAAATTTCTGATTAAATTACCTGAAAAAATAGTTGAACCGGTATTTTCAGAGTTTG
AACGGTCCAATATGCGAATTTGTCAGTGAAATTGGAGGGTTTATGATG
   
Sequence Name 28-K101389-0022-529-D11C-JL72
GenBank Accession LN498773 [GenBank]
Sequencing primer gene specific primer, JL72: 5'-CATCATAAACCCTCCAATTTCACT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K101389-0022-529-D11C-JL72
ACTCTGAAATACCGGTTCACTATTTTTTCGGTAATTTAATCAGACATTTAAAATCTTCTT
CATTTGTTCTTTTATTTTTAACATGATTTTGTTTTTTCAAAAATTGAATAATTTGATTTT
TGAGTATAGAGGAAGCACATGTGCCATGCGATGTTGCTAGAGACGGTGGCTGCGGTGCCG
GGAATGGTCGGGGGGATGCTTTTGCACTTGAAATCTCTCCGGAGGTTCGAACATAGCGGG
GGATGGATCAAAGCTTTGCTCGAATATATACAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTCCGCAGCGT

Last Updated on 06.12.2018 16:11