Sequences from confirmation amplicon


Confirmation sequences for line 529E07 and hit At4g26550 are shown.
Line ID 529E07
Locus At4g26550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13403722
Primers used for PCR JL71 - 8474, show primer details
   
Sequence Name 29-K101383-0022-529-E07C-8409
GenBank Accession LN499219 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K101383-0022-529-E07C-8409
ATTTACATTGAATATATCCTGAATTTGATGACTACAGGGTTTCAAAAGGAGTTAGAGATA
TTCCAGGGAGCTTGCAGTCAGCAACAAGTAGTATGCCTTCAGGAAAAGCGCTCATGTATT
TCGGTTTACTTCTCGCAAGTGGTGTTTTCTTCATCTTCATTGCCTTCACAATGTTTCTTC
CAGTCATGGTGCTTATGCCACAGAAATTTGCCATTTGTTTCACCCTTGGATGTGGATTCA
TCATTGGATCCTTCTTCGCACTTCGTGGCCCTAAAAATCAGCTCGCACACATGTCTTCGA
TGGAGGTATGTTACACTGTTCTATTGTGTGTCTGTCATGCCTTAAATCCAAGAGTGATTT
CCTTCCTCTCTTTTGAATATCTTGTGTACTTCTCCAGAGGCTTCCTTCAACCCTAGGTTT
CATTGCTACCATGGTTGGTACCATATACGTATCANTGGTTCTCCACAGCTACATTCTCTC
GGTGCTCTTCTCAGTGTTACAGGTACTAAACATTTCATCTTCCAATTCTAAGGATATTCC
CTTTAATTATGAGTTTAAGTTTTTCAAAATGAGATATTGCTATGAGCAAGTGGAAAACAA
T
   
Sequence Name 29-K101389-0022-529-E07C-JL71
GenBank Accession LN499220 [GenBank]
Sequencing primer gene specific primer, JL71: 5'-AGGTCCAATATTGTTTTCCACTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K101389-0022-529-E07C-JL71
CTCATAATTAAAGGGAATATCCTAAGAATTGGAAGATGAAATGTTTAGTACCTGTAACAC
TGAGAAGAGCACCGAGAGAATGTAGCTGTGGAGAACCATTGATACGTATATGGTACCAAC
CATGGTAGCAATGAAACCTAGGGTTGAAGGAAGCCTCTGGAGAAGTACACAAGATATTCA
AAAGAGAGGAAGGAAATCACTCTTGGATTTAAGGCATGACAGACACACAATAGAACAGTG
TAACATACCTCCATCGAAGACATGTGTGCGAGCTGATTTTTAGGGCCACGAAGTGCGAAG
AAGGATCCAATGATGAATCCACATCCAAGGGTGAAACAAATGGCAAATTTCTGTGGCATA
AGCACCATGACTGGAAGAAACATTGTGAAGGCAATGAAGATGAAGAAAACACCACTTGCG
AGAAGTAAACCGAAATACATGAGCGCTTTTCCTGAAGGCATACTACTTGTTGCTGACTGC
AAGCTCCCTGGAATATCTCTAACTCCTTTTGAAACCCTGTAGTCATCANATTCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
TTCCGC

Last Updated on 06.12.2018 16:11