Sequences from confirmation amplicon


Confirmation sequences for line 529E10 and hit At1g55610 are shown.
Line ID 529E10
Locus At1g55610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20783271
Primers used for PCR EQ91 - 8474, show primer details
   
Sequence Name 48-K103248-0022-529-E10C-EQ91
GenBank Accession LN506477 [GenBank]
Sequencing primer gene specific primer, EQ91: 5'-AAGAAGGTGAGATTCCCACAGATA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K103248-0022-529-E10C-EQ91
CGGATAGTTGTTGTGAGTAGATCGAGATATTTAACGACGCCGGAAAATCTGAAATAAAAC
TCTCCGGAATCTTATCTGATAAGATGTTGTAAGAGAGATCAACAGTCGTCAAGCTCTGTA
AAGACGACGGAGCAAAACCTAATTTTCCGACGAGCTTGTTGTTGGAGATATTCACCGAAA
CCAGATTCGAACATTTTGAGAAAACGTAATCCACCATTGAATAGTCTGAAATAGAATTCG
AAGACAAATCCAGAACTTGAAGATAACAATCAGAACCAGACGAATCTCCTCCAGAGGAGA
AGTAATTTCCTTGCAAGTAAAGATTCTGGAGATTAGGCAACGCCGTGAGGTTAACTAGGT
TTAGGGTTCCGGTGAGTCCACTGTTTCGGAGATCTAATCCGACGATTCGACCATCGTCAG
AGCAAGAAACCCCTCGCCAAGAGCATGAACCACGACCCGACTCGTATTTCCNGTTACCAA
GAACGTTATTAGGATCAGATTTTACAGAGTTTTGCTTGAACGCCNATNACAGAGCAGGAT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAG
ATGTCCG
   
Sequence Name 44-K103249-0022-529-E10C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K103249-0022-529-E10C-8409
CGATGGTNNCGAATCGTCGGAAATTAGATNNCTCCGAANNACAGTGGANNNTCACCGGAA
CCNCTAANNACCTAGTTAACNCTCGACGGCNNGTTGCCTAATNCTCCANGAANNTCTTTA
CTTGCAAGNGAAATTANNCTTCTCCNNTCTGGAGGAGATNNTCGTCTGGTTCTNNGATTG
TTATNNCTTCAAGTTCNNTGGATTTGTCTNNTCGAATTCTATTTCAGAAACTATTCANNA
TGGTGGATTNNACGTTTTCTCAAAATGGGTTCGAANNTCTGGTTNNTCGGTGAATATNNC
TCCAACAACAANNGCTCGTCGGAAAAAATTAGTGTTTTGGGCTNNNCGTCGTCTTTACNN
AGAGCTTGGACGACTGGGTTGATNNCTCTCTTNNACAACATCTTATCAGAAATAAGAATT
GCCGGAAGAGNNTTTTATTTCAGAATTTTGCCGGCGNNTCGTTGAAATTTATCTCGANNT
CTAACTCANNCAACAACTTATGCCGGGCGACTTTCTCCGAATCNNTCAGTTTCGGTNATT
CTGTGGGAATCTCA

Last Updated on 06.12.2018 16:11