Sequences from confirmation amplicon


Confirmation sequences for line 529F02 and hit At3g27560 are shown.
Line ID 529F02
Locus At3g27560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10212488
Primers used for PCR 36aq - 8474, show primer details
   
Sequence Name 05-K106755-0022-529-F02C-8409
GenBank Accession LN488371 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106755-0022-529-F02C-8409
GCCATTTACATTGAATATTTACTCGAACGAAGAGTTTGAATTGGATCCTAAATGGCTTGT
TGATCCTCGTCATCTCTTTGTTGGTCCTAAGATCGGTGAAGGTGCTCATGCCAAAGTTTA
TGAGGGAAAGTAAGCTTTGACTTACAATCTTTATCATCCATATATACTAAGAATCAAAAG
TTAAAAGTTGTCTCTTTTTATCTATAATCATTGCTTGCTACAGAAATTTCAAATCTTTGT
TTAAGTTTGAAAAAAAAGAACTTAGTCTTGAAAAAAGTTTGACTCTTTGCGTATATGAAG
CACAAGAGAATCTTTTATTGTCTGGTTCAGTTACTTATTAGGCTAATTGGTTTAATCTTT
CTTTCTAGTATTTATTGAAGCTTATCTGTTTCTGGAGATGCTTGAAGATTGTTGTTTGAT
GATGNAAAAAT
   
Sequence Name 21-K106756-0022-529-F02C-36aq
GenBank Accession LN495679 [GenBank]
Sequencing primer gene specific primer, 36aq: 5'-ACATTTTCAATCCATCAAACAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K106756-0022-529-F02C-36aq
AAGCTTCATAATACTAGAAAGAAAGATTAAACCAATTAGCCTAATAAGTAACTGAACCAG
ACAATAAAAGATTCTCTAGTGCTTCATATACGCAAAGAGTCAAACTTTTTTCAAGACTAA
GTTCTTTTTTTTCAAACTTAAACAAAGATTTGAAATTTCTGTAGCAAGCAATGATTATAG
ATAAAAAGAGACAACTTTTAACTTTTGATTCTTAGTATATATGGATGATAAAGATTGTAA
GTCAAAGCTTACTTTCCCTCATAAACTTTGGCATGAGCACCTTCACCGATCTTAGGACCA
ACAAAGAGATGACGAGGATCAACAAGCCATTTAGGATCCAATTCAAACTCTTCGTTCGAG
TAAATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCC

Last Updated on 06.12.2018 16:11