Sequences from confirmation amplicon


Confirmation sequences for line 529G04 and hit At1g13210 are shown.
Line ID 529G04
Locus At1g13210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4513106
Primers used for PCR CM23 - 8474, show primer details
   
Sequence Name 45-K030903-022-529-G04C-8409
GenBank Accession LN505421 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K030903-022-529-G04C-8409
TCGAGAATTCATACGTCTCTACATAACAAAATCGAATCTTCGTAGCTAGATCGATAACAG
CAAAAGATCCGCCGGAAAAAAACTCGTCTTTTTCAACTCTTACTATATTTCCGACTTTAA
GAATCCCTCCACCCTTCTCGCCGGAATATTCCATTTCCGTCGTGAACCTTCACTTTCCTA
TTATTCATTTCGATGTCCTAAATATAGAAGAAGAAAAAAACGAAACTTTGATTTAAAAAT
TGAAACTTTATAATTTGAGAGAA
   
Sequence Name 46-K030903-022-529-G04C-CM23
GenBank Accession LN505764 [GenBank]
Sequencing primer gene specific primer, CM23: 5'-GCTCAAATCGAATTTCTCTCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K030903-022-529-G04C-CM23
CCGGCGGATCCTCTGCCGTTATCATCTAGCTACGAAGATTCGATTTCGTTACGTAGAGAC
TACGAATCTCGACGGAGAAACGAATCTGAAAGTGAAACAAGCACTATCAGGATATATTCA
ATCGTAAACGGCTTCACGTCCGGGAAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTC
TA

Last Updated on 06.12.2018 16:11