Sequences from confirmation amplicon


Confirmation sequences for line 530B04 and hit At1g31230 are shown.
Line ID 530B04
Locus At1g31230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11161854
Primers used for PCR 30cs - 8474, show primer details
   
Sequence Name 28-K109134-0022-530-B04C-30cs
GenBank Accession LN498883 [GenBank]
Sequencing primer gene specific primer, 30cs: 5'-ATCCACTATCAGCTCACCCTTCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K109134-0022-530-B04C-30cs
TCGTCGCAATCCTTTCACAGGAGCGTCTAATTTGAATCCATCTTCATCATCAATCTCCTG
GCATATCATTGTTCCAGGGGCAGAGAGGTTGAAAATATTCCTTATTACAATTGGAATGTC
ATATTTCATCACTGGAATAATGGTCCGAGGATGTAAAACGTTTGCCCCAAAGTAAGACTG
CGCAGGCATATGAACATGAATGTTAGGTGTAAACAACGATTCTGGAAAACTAGGCATTAA
ACAAGTGCAAGAGGTTGGAAATTTACCATTTCCCAAGCCTCTTGATAAGAAAGAGTCTTC
AGCACAACAGCTTCACTAACTGCACCGCACAAATTCATTAGCATAACACAGACAAACATG
ATGAGGAACACACACATATCATGGCTCAATAACTAGTCCTAGCTATATGGCAGTTAATGT
AGGCTTGCACATACCTTTCCTGGGATCTGCACTGTACACACCATCAACATCTGTCCAGAT
NGTGAGTTGGTGAGATCTACATGGATCAGCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTTCAATTCAAAAATGT
   
Sequence Name 12-K109133-0022-530-B04C-8409
GenBank Accession LN491617 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K109133-0022-530-B04C-8409
ATTTACATTGCTGATCCATGTAGATCTCACCAACTCACAATCTGGACAGATGTTGATGGT
GTGTACAGTGCAGATCCCAGGAAAGGTATGTGCAAGCCTACATTAACTGCCATATAGCTA
GGACTAGTTATTGAGCCATGATATGTGTGTGTTCCTCATCATGTTTGTCTGTGTTATGCT
AATGAATTTGTGCGGTGCAGTTAGTGAAGCTGTTGTGCTGAAGACTCTTTCTTATCAAGA
GGCTTGGGAAATGGTAAATTTCCAACCTCTTGCACTTGTTTAATGCCTAGTTTTCCAGAA
TCGTTGTTTACACCTAACATTCATGTTCATATGCCTGCGCAGTCTTACTTTGGGGCAAAC
GTTTTACATCCTCGGACCATTATTCCAGTGATGAAATATGACATTCCAATTGTAATAAGG
AATATTTTCAACCTCTCTGCCCCTGGAACAATGATATGCCGGCAGATTGATGATGAAGAT
GGATTCAAATTAGACGCTCCTGTGAAAGGATTTGCGACGATTGACAATTTGGCTCTTGTC
AATGTAGAAGGGTGAGCTGATAGTGGA

Last Updated on 06.12.2018 16:11