Sequences from confirmation amplicon


Confirmation sequences for line 530C12 and hit At3g56360 are shown.
Line ID 530C12
Locus At3g56360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20897329
Primers used for PCR MN35 - 8474, show primer details
   
Sequence Name 13-K031753-022-530-C12C-8409
GenBank Accession LN491919 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K031753-022-530-C12C-8409
CATTTACAATTGAATATAGCACCAACCACGAATTATTCAACTGTGACATAGCCGTTTCCC
TAAAACTCTTGACCTTAACCAAACAGAAATGGGTGCAAACCGTACCCATACCCGACCGGA
CCCGGGTCGTTTTCAATAGATAACAAACCGGCCCAGTATCCGATCCTCTTCCTTGCGCCA
CAATCCCCCACGCGCTTGTATCTTCCCCACACGCGCCACCATCGTGATCATCATCATCTG
CTATGTCACCGTCTGCGAGCTTCTCCACCAATATAACTCCTTCCGGCGTCCTCTCTTCTC
CTCTACCCCACTTTCCTTCCATCTCTGTCCAATCCTCCGCCTTTGGATTCGTTGCCGTCG
TCGTCAAACCACCATTAGCCTCCGCGTATAAATGCATCAATAACGGCGACTCTCTAAGA
   
Sequence Name 14-K031753-022-530-C12C-MN35
GenBank Accession LN492391 [GenBank]
Sequencing primer gene specific primer, MN35: 5'-TCTTAGAGAGTCGCCGTTATTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K031753-022-530-C12C-MN35
ACGGCACGAATCCAAGGCGGAGGATTGGACAGAGATGGAAGGAAAGTGGGGTAGAGGAGA
AGAGAGGACGCCGGAAGGAGTTATATTGGTGGAGAAGCTCGCAGACGGTGACATAGCAGA
TGATGATGATCACGATGGTGGCGCGTGTGGGGAAGATACAAGCGCGTGGGGGATTGTGGC
GCAAGGAAGAGGATCGGATACTGGGCCGGTTTGTTATCTATTGAAAACGACCCGGGTCCG
GTCGGGTATGGGTACGGTTTGCACCCATTTCTGTTTGGTTAAGGTCAAGAGTTTTAGGGA
AACGGCTATGTCACAGTTGAATAATTCGTGGTTGGTGCTATATTCAATTGTAAATGGCTT
CATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGA
AAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAA
AATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGC
AATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAA
ATGGG
Primers used for PCR 02bd - 8474, show primer details
   
Sequence Name 77-K118629-0022-530-C12C-02bd
GenBank Accession LN513611 [GenBank]
Sequencing primer gene specific primer, 02bd: 5'-GGCTTTGCACTATGATCACTAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K118629-0022-530-C12C-02bd
CTTTTGCTATTGTGGTGTAATGAATGCATAATTGCATATAGATCTTACACGGATTAAGTT
GTCTACTTGTCTTGATTCAAATCCTCTATACTACAATAGCAACAAAACTAACAATGTTCT
TTGATTAAACAAATCACTATAACCAACCAACTTTGACCATTTTATTTACATTTATATCAC
CAATCCAAATTCCTAAAAAATGTTCTGTATTAATAACAAATGTAATTTGATCATCCATAA
GGGTTTCATAATCAAAACCCAAAGAGTATAAATAATTTACAGTGCAAATCCTTTGACAAT
CACTGCTCATTGACCAGTCTACATGGCTTCATGTCCGTATATATTCAATTGACCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCACAAGATTATAAT
   
Sequence Name 58-K118628-0022-530-C12C-8409
GenBank Accession LN509835 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K118628-0022-530-C12C-8409
AATTGAATATATCCTGGTCAATTGAATATATACGGACATGAAGCCATGTAGACTGGTCAA
TGAGCAGTGATTGTCAAAGGATTTGCACTGTAAATTATTTATACTCTTTGGGTTTTGATT
ATGAAACCCTTATGGATGATCAAATTACATTTGTTATTAATACAGAACATTTTTTAGGAA
TTTGGATTGGTGATATAAATGTAAATAAAATGGTCAAAGTTGGTTGGTTATAGTGATTTG
TTTAATCAAAGAACATTGTTAGTTTTGTTGCTATTGTAGTATAGAGGATTTGAATCAAGA
CAAGTAGACAACTTAATCCGTGTAAGATCTATATGCAATTATGCATTCATTAACACCACA
ATAAGCAAAAGTGTAGGTGGATTTAAAACTTCTGTTAGTGATCATAGTGCAAAGCC

Last Updated on 06.12.2018 16:11