Sequences from confirmation amplicon


Confirmation sequences for line 530E08 and hit At3g01930 are shown.
Line ID 530E08
Locus At3g01930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:320593
Primers used for PCR 51gp - 8474, show primer details
   
Sequence Name 45-K112250-0022-530-E08C-51gp
GenBank Accession LN505577 [GenBank]
Sequencing primer gene specific primer, 51gp: 5'-TGCAACTGAGCAATTCTCTTATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K112250-0022-530-E08C-51gp
TGAGACATCTCTTCTTTTGGTTTCTCGTCTTCCACTTCACTATATAAGCTCAGGACCATG
GTCAGGGGTGGTGGACTGTCCCGGATCCTGACCTTGCTGATCGCCAAGGAGAGGTTCTTC
GAGCGTGTCACAGGGGTCGGTTGATGCTGTGAAGCAACTCGTTGCGATTGGTATGAAGAT
GGGTACAAGAAGAATGGCAAACAAGACAACGGTGAAAGCAATGATGATTGAATGGCTAAG
ATCAATGAAATCTTCAACAAGCATCACTGCCATGAGATAGGCAGCAAGTAGAATGCAGAC
GGCATAGATGACGGTGAAGCTGGTGGCATCAGAAGAACGGATCTGTCTGTGACCACCGAC
GGGTCTGATGAAGAACATGAGAGGAACAACAACAACTGAAGGGGCAACAGCAACCATGAA
GATGAGAGGATGGCACCGCCGATACAGGCCAGGATACAGGCCAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 35-K112249-0022-530-E08C-8409
GenBank Accession LN501882 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K112249-0022-530-E08C-8409
TTACATTGAATTATCCTGGCCTGTATCCTGGCCTGTATCGGCGGTGCCATCCTCTCATCT
TCATGGTTGCTGTTGCCCCTTCAGTTGTTGTTGTTCCTCTCATGTTCTTCATCAGACCCG
TCGGTGGTCACAGACAGATCCGTTCTTCTGATGCCACCAGCTTCACCGTCATCTATGCCG
TCTGCATTCTACTTGCTGCCTATCTCATGGCAGTGATGCTTGTTGAAGATTTCATTGATC
TTAGCCATTCAATCATCATTGCTTTCACCGTTGTCTTGTTTGCCATTCTTCTTGTACCCA
TCTTCATACCAATCGCAACGAGTTGCTTCACAGCATCAACCGACCCCTGTGACACGCTCG
AAGAACCTCTCCTTGGCGATCAGCAAGGTCAGGATCCGGGACAGTCCACCACCCCTGACC
ATGGTCCTGAGCTTATATTTAGTGAAGTGGAAGACGAGAAACCAAAAGAAGTAGATTTGC
TTCCTGCAGTGGAGAGACATAAGAGA

Last Updated on 06.12.2018 16:11