Sequences from confirmation amplicon


Confirmation sequences for line 531G01 and hit At1g32970 are shown.
Line ID 531G01
Locus At1g32970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11948811
Primers used for PCR MZ90 - 8474, show primer details
   
Sequence Name 69-K027350-022-531-G01C-8409
GenBank Accession LN512112 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K027350-022-531-G01C-8409
GATTTCATCATCAGTTTGCATGATCTGAGTTCTCACAGACACANGGAATAACCAAAGTAG
TATCCAGTGTTCACTATATGGTTCGTTAAGACACGAACCTTAAACGAGAGCTTCCTGGTA
TAAGCATTGAACACTAATGTGCTTGGTGTCACGGTCACGTTAACACCCATTGGAGCCTCA
ATCACAGGCTTATAAACTGACCCTACTGGTCCAACGTTGGTAACCGTTCTAGTGATAGTA
ACTTCTTTTGCAAGGTTTGGGATTGTGATTGAAGGCAAATTGAGATCAAGAACTGAAGGT
TTAGGGTTTGCACATACTGTCTTCTTGCTGACGAGTCCAGTAATAGATGAATCTGTGTAA
CCAACTGAACACAAGTAGAGGATGTAATCTTTAACTCCCATATCGTAAACAAGACCGGGG
TTTGCTGCTTTCTCTGAATTCACAACGCCTCCTCCGTAGTCAAATGGATCACT
   
Sequence Name 70-K027350-022-531-G01C-MZ90
GenBank Accession LN512296 [GenBank]
Sequencing primer gene specific primer, MZ90: 5'-CAAACCGTAAATTAGCTGATCCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K027350-022-531-G01C-MZ90
AGCAGCAAACCCCNGGTCTTGTTTACGAATATGGGAGTTAAAGATTACATCCTCTACTTG
TGTTCAGTTGGTTACACAGATTCATCTATTACTGGACTCGTCAGCAAGAAGACAGTATGT
GCAAACCCTAAACCTTCAGTTCTTGATCTCAATTTGCCTTCAATCACAATCCCAAACCTT
GCAAAAGAAGTTACTATCACTAGAACGGTTACCAACGTTGGACCAGTAGGGTCAGTTTAT
AAGCCTGTGATTGAGGCTCCAATGGGTGTTAACGTGACCGTGACACCAAGCACATTAGTG
TTCAATGCTTATACCAGGAAGCTCTCGTTTAAGGTTCGTGTCTTAACGAACCATATAGTG
AACACTGGATACTACTTTGGTTATTCCTGTGTCTGTGAGAACTCAGATCATGCAAACTGA
TGATGAAATCTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA
GCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTT
ATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTA

Last Updated on 06.12.2018 16:11