Sequences from confirmation amplicon


Confirmation sequences for line 532G01 and hit At1g49640 are shown.
Line ID 532G01
Locus At1g49640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:18376351
Primers used for PCR KP20 - 8474, show primer details
   
Sequence Name 03-K030138-022-532-G01C-8409
GenBank Accession LN487357 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K030138-022-532-G01C-8409
CATTTACAATTGAATATATCCTGTGAAGGAGATTGTATGATGTAGTTATGGTAAACCGGA
GAGAAAGGAGATTGTATGATGTAAGCTCCGCCGTGAAAGTAGATGAGTAGAGGTATTTTG
TTACCGGCGGTGTCGAGTTTTCTAGATTTGTTGGGGAGAAACATACGGACGGAGAGGTTG
TGGTCCGATGAGTACATGACGTCTTTCGAAACGACATCGTTTTGTGGGTTTAAAGACGTT
GGTTTGATGTCGTTGCCGCTAAGCCGCTCGACTCGGCCGTTCTTATGAATTCGGATGAAT
GGTAGATGATGCTCTGTGGTTAGATCAGATTCCATCTTCTTTGGAAAAGAGAGTGAAGAA
CAGAAACAGAGTAATATGTATGTGAGTTTACTATATGTATGAGTATACACTGATTCATCC
AATCAAACCCTAGACAAGTA
   
Sequence Name 03-K030138-022-532-G01C-KP20
GenBank Accession LN487358 [GenBank]
Sequencing primer gene specific primer, KP20: 5'-GTACTTGTCAGGGTTTGATTGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K030138-022-532-G01C-KP20
ACTCCATACATATTACTCTGTTTCTGTTCTTCCTCTCTTTTCCAAAGAAGATGGAATCTG
ATCTAACCACAGAGCATCATCTACCATTCATCCGAATTCATAAGAACGGCCGAGTCGAGC
GGCTTAGCGGCAACGACATCAAACCAACGTCTTTAAACCCACAAAACGATGTCGTTTCGA
AAGACGTCATGTACTCATCGGACCACAACCTCTCCGTCCGTATGTTTCTCCCCAACAAAT
CTAGAAAACTCGACACCGCCGGTAACAAAATACCTCTACTCATCTACTTTCACGGCGGAG
CTTACATCATACAATCTCCTTTCTCTCCGGTTTACCATAACTACATCATACAATCTCCTT
CACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT
TATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11