Sequences from confirmation amplicon


Confirmation sequences for line 533B08 and hit At5g10740 are shown.
Line ID 533B08
Locus At5g10740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3394625
Primers used for PCR BK77 - 8474, show primer details
   
Sequence Name 28-K105013-0022-533-B08C-BK77
GenBank Accession LN498839 [GenBank]
Sequencing primer gene specific primer, BK77: 5'-CATGTGATGTTATCTGCACTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K105013-0022-533-B08C-BK77
TTTTTCGCTGAGTCTCGGGATCTTCAACTTCCTTTACCATTGCAACTGCTGCCTGCAAAA
TATGATCATGTATTATTGTAATTAAATTGTTGGAGTAGGAAATAGAAGATGTTAAAGAAG
CAATTCACTTCACAGACCTCATTAGAGAAAACATCCCACAGTCCATCACTTGCTAGAATC
AAAAACTCAAGAGTATCATCAATCTTTTCTTCCTGTATTCAATGACAAAAGTTTGAGAAA
TTTGTCAGCTGGAGACAAAAGGGGACAAAAAGATTATGCAATGTGATTCTTAGAGAAGTG
ATGAAAAGACCTGGATTTCTGGATCAGCAACAACATATTGCTTCAGAAGCCGATCACCAA
ACGCACGAGAAACTGCAAGAACTCCTCCTACCCTCCAAGTTCCTATTTAGTTTATGAATG
AACACACCCCTATAATTAGGACTATATGTTGAAAAAAGCATGAAGCAGCAGAAAATATAG
TCCTATAGTTTCTTGCTACTTACCTGCCCACATCACAAATCCACCAGCATTCTCAATTGT
AAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGG
AGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 12-K105014-0022-533-B08C-8409
GenBank Accession LN491570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K105014-0022-533-B08C-8409
ATTGAGAATGCTGGTGGATTTGTGATGTGGGCAGGTAAGTAGCAAGAAACTATAGGACTA
TATTTTCTGCTGCTTCATGCTTTTTTCAACATATAGTCCTAATTATAGGGGTGTGTTCAT
TCATAAACTAAATAGGAACTTGGAGGGTAGGAGGAGTTCTTGCAGTTTCTCGTGCGTTTG
GTGATCGGCTTCTGAAGCAATATGTTGTTGCTGATCCAGAAATCCAGGTCTTTTCATCAC
TTCTCTAAGAATCACATTGCATAATCTTTTTGTCCCCTTTTGTCTCCAGCTGACAAATTT
CTCAAACTTTTGTCATTGAATACAGGAAGAAAAGATTGATGATACTCTTGAGTTTTTGAT
TCTAGCAAGTGATGGACTGTGGGATGTTTTCTCTAATGAGGTCTGTGAAGTGAATTGCTT
CTTTAACATCTTCTATTTCCTACTCCAACAATTTAATTACAATAATACATGATCATATTT
TGCAGGCAGCAGTTGCAATGGTAAAGGAAGTTGAAGATCCCGAGGACTCAGCGAAAAAAC
TGGTGGGTGAAGCAATAAAGAGAGGAAGTGCAG

Last Updated on 06.12.2018 16:11