Sequences from confirmation amplicon


Confirmation sequences for line 533D01 and hit At3g61850 are shown.
Line ID 533D01
Locus At3g61850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22896023
Primers used for PCR DC81 - 8474, show primer details
   
Sequence Name 08-K032598-022-533-D01C-DC81
GenBank Accession LN489627 [GenBank]
Sequencing primer gene specific primer, DC81: 5'-CTTTGTTGAAGTTCATGGATGCTA-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K032598-022-533-D01C-DC81
AAGATATCTCTTTGCTAATCTGTTTGTTTCTAGCTTCTCCCTGACTCTTACGCTCTCTCT
CTCTCTCTCTCTCTCTTTTTATCTCTCTACTCACATAAATATATACATGTGTGTGTATGC
ATGTTTATATGTATGTATGAAACCAGTAGTGGTTATACAGATAGTCTATATAGAGATATC
AATATGATGTGTTTTAATTTAGACTTTTTATATATCCGTTTGAAACTTCCGAAGTTCTCG
AATGGAGTTAAGAAAGTTTTGTTCTCTACAAGTTCAATTTTTCTTGTCATTAATTATAAA
ACTCTGATAACTAATGGATAAAAAAGGTATGCTTTGTTAGTTACCTTTTGTTCTTGGTGC
TCAGGTCTTACCATTTTTTTCCTAAATTTTAATTAGTCTCCTTTCTTTAATTAATTTTAT
GTTAACGCACTGACGATTTAACGTTAACAAAAAAACCTAGATTCTTTTTCTTTTCAATAG
AGCATAATTATTACTTGGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATGGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAATCTTTATTTCGACGTGTCTACTC
   
Sequence Name 08-K032598-022-533-D01C-8409
GenBank Accession LN489626 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K032598-022-533-D01C-8409
CATTTACATTGATCCAGTAATAATTATGCTCTATTGAAAAGAAAAAGAATCTAGGTTTTT
TTGTTAACGTTAAATCGTCAGTGCGTTAACATAAAATTAATTAAAGAAAGGAGACTAATT
AAAATTTAGGAAAAAAATGGTAAGACCTGAGCACCAAGAACAAAAGGTAACTAACAAAGC
ATACCTTTTTTATCCATTAGTTATCAGAGTTTTATAATTAATGACAAGAAAAATTGAACT
TGTAGAGAACAAAACTTTCTTAACTCCATTCGAGAACTTCGGAAGTTTCAAACGGATATA
TAAAAAGTCTAAATTAAAACACATCATATTGATATCTCTATATAGACTATCTGTATAACC
ACTACTGGTTTCATACATACATATAAACATGCATACACACACATGTATATATTTATGTGA
GTAGAGAGATAAAAAGAGAGAGAGAGAGAGAGAGAGAGCGTAAGAGTCAGGGAGAAGCTA
GAAACAAACAGATATAGCAAAGAGATATCTTTTTACGTACCTGAGTCCACTTCGTAGCAT
CCATG

Last Updated on 06.12.2018 16:11