Sequences from confirmation amplicon


Confirmation sequences for line 533G02 and hit At5g45820 are shown.
Line ID 533G02
Locus At5g45820 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18587836
Primers used for PCR JK26 - 8474, show primer details
   
Sequence Name 33-K024725-022-533-G02C-8409
GenBank Accession LN500841 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K024725-022-533-G02C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCCGCATAACCTTTCTTGCCT
ATCACTTCAGGTGCCACGTAAGCAGGTGTTCCACATGTCGTGTGAAGCAAGCCATCTTGC
TGCTTCGACTCCCTCAACGCGCTAAGGCCAAAATCCGATATTTTCAAATCGCCGTTTTCG
TCTAGAAGAAGATTCTCCGGTTTGAGATCGCGGTGGTAAACTCCGCGGCTATGGCAATAA
TCGATTGCTCCGATCAATTGCTGGAAATATTTTCGAGCAATGTTTTCTTTAAGCTTTCCT
TTAGAGACTTTATCAAAAAGCTCACCGCCTTTAACGTATTCCATAGCGAAATAGATCTTT
GTCTTGCTCGCCATTACTTCATGGAGGAAGACGACGTGGGGGTGACGAACGAGACGCATC
ACTGATAT
   
Sequence Name 34-K024725-022-533-G02C-JK26
GenBank Accession LN501263 [GenBank]
Sequencing primer gene specific primer, JK26: 5'-TCAAACGAGAAATATCAGTGATGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K024725-022-533-G02C-JK26
TCGTTCGTCACCCCCACGTCGTCTTCCTCCATGAAGTAATGGCGAGCAAGACAAAGATCT
ATTTCGCTATGGAATACGTTAAAGGCGGTGAGCTTTTTGATAAAGTCTCTAAAGGAAAGC
TTAAAGAAAACATTGCTCGAAAATATTTCCAGCAATTGATCGGAGCAATCGATTATTGCC
ATAGCCGCGGAGTTTACCACCGCGATCTCAAACCGGAGAATCTTCTTCTAGACGAAAACG
GCGATTTGAAAATATCGGATTTTGGCCTTAGCGCGTTGAGGGAGTCGAAGCAGCAAGATG
GCTTGCTTCACACGACATGTGGAACACCTGCTTACGTGGCACCTGAAGTGATAGGCAAGA
AAGGTTATGCGGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAAC
GACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG
AAAT

Last Updated on 06.12.2018 16:11