Sequences from confirmation amplicon


Confirmation sequences for line 534A02 and hit At4g02120 are shown.
Line ID 534A02
Locus At4g02120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:943412
Primers used for PCR MZ59 - 8474, show primer details
   
Sequence Name 13-K025300-022-534-A02C-8409
GenBank Accession LN491872 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K025300-022-534-A02C-8409
CCATGTAGATTTCCCGGACATGAAGCCATTTCCCTGTGTTAATATATCACCAAGAGTGAG
AGAATGAACGGACTCCATTGATAGGTTTCTGAATACATACATGTTTGGAATACATACTTC
TGGCATAAATATAACAACAGGGTCAGATGTCTGAGCATCGAACTCAGTGCTATTTGCTCT
TTCCAAACCCAAAACCTGTAACCAATGTCATAGAATAAGAAGAAGAAGAACGTTACCAAA
GTACAGAGCAAGTTGAAGTTATTCCAAGACATTTCTTACAGATCTTGCAAACTCAATTAC
TGCAATTTGCATCCCCAAGCAGATCCCAAGATAAGGAATTTTGTTCTCTCTAGCGTATTT
GGCTGCTAAAACCATTCCACTCACACCACGATCTCCAAAACCACCAGGAACCAGAATGCA
TTCTGCGCTCTGCAAGTGGAGAGTTTATGAACTAAATCAAATCAGAGAATTCTAAGACTG
AAATTCTCACCTTCA
   
Sequence Name 14-K025300-022-534-A02C-MZ59
GenBank Accession LN492342 [GenBank]
Sequencing primer gene specific primer, MZ59: 5'-TTGGAAGATCTTGAAGGTGAGAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K025300-022-534-A02C-MZ59
AGTCTTAGAATTCTCTGATTTGATTTAGTTCATAAACTCTCCACTTGCAGAGCGCAGAAT
GCATTCTGGTTCCTGGTGGTTTTGGAGATCGTGGTGTGAGTGGAATGGTTTTAGCAGCCA
AATACGCTAGAGAGAACAAAATTCCTTATCTTGGGATCTGCTTGGGGATGCAAATTGCAG
TAATTGAGTTTGCAAGATCTGTAAGAAATGTCTTGGAATAACTTCAACTTGCTCTGTACT
TTGGTAACGTTCTTCTTCTTCTTATTCTATGACATTGGTTACAGGTTTTGGGTTTGGAAA
GAGCAAATAGCACTGAGTTCGATGCTCAGACATCTGACCCTGTTGTTATATTTATGCCAG
AAGTATGTATTCCAAACATGTATGTATTCAGAAACCTATCAATGGAGTCCGTTCATTCTC
TCACTCTTGGTGATATATTAACACAGGGAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAA
CGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCG
GAAATCTTTATTTCGACGTGTCTACA

Last Updated on 06.12.2018 16:11