Sequences from confirmation amplicon


Confirmation sequences for line 534C01 and hit At3g17180 are shown.
Line ID 534C01
Locus At3g17180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5857624
Primers used for PCR MZ58 - 8474, show primer details
   
Sequence Name 17-K025301-022-534-C01C-8409
GenBank Accession LN493703 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K025301-022-534-C01C-8409
AGATTTCCCGGACATGAAGCCATTTACAATTGAATATAACACATGATTTCAGTGTTTCTT
TTTAATCTCTTTGAAAAGCTTTTTTAAAATTCAATTATTTAGGATTTTCTTTCACTGTAC
TTAAGTCTAAAACTTGTCAGTATAGGTGGGAAATCCATTGACGGACGATGAGTACGATAA
CAAAGGGATTCTAGAATACGCATGGAGCCATGCAGTGATCTCTGATCATCTTTACGACTC
CGCAAAACATAACTGCGACTTCAAGTCTTCGAACTGGTCAGAGCCATGCAACGTTGCTAT
GAACACAGTGTTCACAAAATACAAAGAGATTGATATTTATAACATATATGCTCCCAAGTG
CATATCTAATAGCTCATCAGGAGCTTCCTACTTAGGTT
   
Sequence Name 18-K025301-022-534-C01C-MZ58
GenBank Accession LN494151 [GenBank]
Sequencing primer gene specific primer, MZ58: 5'-TTGACTCCGAAACCTAAGTAGGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K025301-022-534-C01C-MZ58
GCTCCTGATGAGCTATTAGATATGCACTTGGGAGCATATATGTTATAAATATCAATCTCT
TTGTATTTTGTGAACACTGTGTTCATAGCAACGTTGCATGGCTCTGACCAGTTCGAAGAC
TTGAAGTCGCAGTTATGTTTTGCGGAGTCGTAAAGATGATCAGAGATCACTGCATGGCTC
CATGCGTATTCTAGAATCCCTTTGTTATCGTACTCATCGTCCGTCAATGGATTTCCCACC
TATACTGACAAGTTTTAGACTTAAGTACAGTGAAAGAAAATCCTAAATAATTGAATTTTA
AAAAAGCTTTTCAAAGAGATTAAAAAGAAACACTGAAATCATGTGTTATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGT
TATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAG
GCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC
A

Last Updated on 06.12.2018 16:11