Sequences from confirmation amplicon


Confirmation sequences for line 534D06 and hit At5g15120 are shown.
Line ID 534D06
Locus At5g15120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4899554
Primers used for PCR 19kt - 8474, show primer details
   
Sequence Name 27-K113817-0022-534-D06C-19kt
GenBank Accession LN498476 [GenBank]
Sequencing primer gene specific primer, 19kt: 5'-CTCTAATGGTGGTCCTGGTGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K113817-0022-534-D06C-19kt
ACGCTCGAGAGATTCTTGGTATCAATTTTTATCAGTTTTCTGAATTCCTTGTTCTGGGTT
TCCATAAAGTTTTGATTTTTGATTGGATTTTGGTTTATAGACGATATGAAACCAGAGGAT
GTCGGTTTAACTCCGACCATGCCGTATTTCCGACCAAACTCCGGAGTCGAAGCTCGGTCT
TCGCCACCAATAACGTATCTGCATCTACACCAGTGTGATCAATTCTCGGTAAATTCCACT
ACAAACACTCTCTCACTTGTATTGTTCTCTTTCTTGTGGTATAAGTAATCATGGTTAGGG
TTTTATCGCAGATTGGGATTTTCTGTTTGCCACCTTCTGGTGTTATTCCTCTTCATAACC
ATCCAGGGATGACAGTTTTTAGTAAGCTTCTCTTTGGTACAATGCACATCAAGTCATATG
ATTGGGTGGTTGATGCTCCAAGTAAGTTTACTTTCGTCCCAAGAAAACTTTTGTGAGCTC
TGAAACTTGTATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCGCA
   
Sequence Name 01-K113816-0022-534-D06C-8409
GenBank Accession LN486654 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K113816-0022-534-D06C-8409
CATTGATATACAGTTTCAGAGCTCCAAAAGTTTTCTTGGGACGAAAGTAAACTTACTTGG
AGCATCAACCACCCAATCATATGACTTGATGTGCATTGTACCAAAGAGAAGCTTACTAAA
AACTGTCATCCCTGGATGGTTATGAAGAGGAATAACACCAGAAGGTGGCAAACAGAAAAT
CCCAATCTGCGATAAAACCCTAACCATGATTACTTATACCACAAGAAAGAGAACAATACA
AGTGAGAGAGTGTTTGTAGTGGAATTTACCGAGAATTGATCACACTGGTGTAGATGCAGA
TACGTTATTGGTGGCGAAGACCGAGCTTCGACTCCGGAGTTTGGTCGGAAATACGGCATG
GTCGGAGTTAAACCGACATCCTCTGGTTTCATATCGTCTATAAACCAAAATCCAATCAAA
AATCAAAACTTTATGGAAACCCAGAACAAGGAATTCAGAAAACTGATAAAAATTGTATAC
CAAGAATCTCTCGTAGCTGTTGGATTTTATCTTCAGAAGGAATAACACCAGGACCACCAT
TAGA

Last Updated on 06.12.2018 16:11