Sequences from confirmation amplicon


Confirmation sequences for line 535C09 and hit At3g33530 are shown.
Line ID 535C09
Locus At3g33530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:14093369
Primers used for PCR 22sf - 8474, show primer details
   
Sequence Name 65-K116453-0022-535-C09C-22sf
GenBank Accession LN511473 [GenBank]
Sequencing primer gene specific primer, 22sf: 5'-CAACACATTTAGAAGGATCGGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K116453-0022-535-C09C-22sf
ATCAATTATTATTTCCTATTATTGGTTACTAGTCGTTGTAACTACTCCTATCTCTCTATC
TACTCAGGGTATTGCAAAGGTGGGCTGATCATGTTCTCCATGCGGAGCACAACGTCTGGC
GGTACTTTATCATCATGCTCACATTCAATACAGTTTAACTTAGAAAATTTAAATGTGACA
TAAATTCCTCAAACAGTTCCGATGATGAGAAAAGAATTTCATTTTATTAATGCAGGGCCC
TTATTTTATATGTGGCTGCTGGTTCCCTGCAAGAGGCGCTTGCAGCTCTCAGAGAGGTGC
AACAGCCTGATACAGTAGCCATGTTTGTCCTCGCGTGTCAGGAGATTCATTCAGAAATCA
TAACCGAGCTATCAACCCAAGACGAGGAGTCTGAATCTGCTTCTGGTGATGGCACAGTAC
CGCTGCCCGATCTGCCTGGTCTTGAATCAGGGAAGGAAGAAGTGAGTAAATATGATGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATATCCGCA
   
Sequence Name 32-K116452-0022-535-C09C-8409
GenBank Accession LN500726 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K116452-0022-535-C09C-8409
CATATTTACTCACTTCTTCCTTCCCTGATTCAGACCAGGCAGATCGGGCAGCGGTACTGT
GCCATCACCAGAAGCAGATTCAGACTCCTCGTCTTGGGTTGATAGCTCGGTTATGATTTC
TGAATGAATCTCCTGACACGCGAGGACAAACATGGCTACTGTATCAGGCTGTTGCACCTC
TCTGAGAGCTGCAAGCGCCTCTTGCAGGGAACCAGCAGCCACATATAAAATAAGGGCCCT
GCATTAATAAAATGAAATTCTTTTCTCATCATCGGAACTGTTTGAGGAATTTATGTCACA
TTTAAATTTTCTAAGTTAAACTGTATTGAATGTGAGCATGATGATAAAGTACCGCCAGAC
GTTGTGCTCCGCATGGAGAACATGATCAGCCCACCTTTGCAATACCCTGAGTAGATAGAG
AGATAGGAGTAGTTACAACGACTAGTAACCAATAATAGGAAATAATAATTAGATCTAGAA
GTAAACCGTAGAAGAAAAGACCTGGCATAATCCGATCCTCAAAAATGTGTTG

Last Updated on 06.12.2018 16:11