Sequences from confirmation amplicon


Confirmation sequences for line 535G10 and hit At3g27280 are shown.
Line ID 535G10
Locus At3g27280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10077721
Primers used for PCR CV10 - 8474, show primer details
   
Sequence Name 14-K030753-022-535-G10C-8409
GenBank Accession LN492385 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K030753-022-535-G10C-8409
TGAGCCATTTACAATTGAATATATAGAGCTCAATGTTAAATTCCCTGGCACGCTTGATAA
GAGCATCACGAACAAGTGCTGATACTTGAGGACGCTCAGTGAGGAGCTGATCAGCATTGA
AATTAGCAACCACAGCTTTCAAGACCTCATTTCCAATCGATGGAAGAACCTTCTCATCGT
ATTCAAGACCCAATGTTTGGAAAATGTACGGGAGACGCGAAACCTTCACAGTAAAATAAC
CACAACAACTTATTCAATCCCTCAAACAATCGAAGCCAGCATAGATATGCACCAAAAGCT
TCTATAATAAGAGTAACATTCATCGAAGAAACCAAATTACACATAAGGATTATCTTCAAC
TCTAGATCTTGTACACAAACTTCAAATTCCACGAAATCAAACACAGAATCGATAAATGAT
TGCTAAATCGAACGAACACGATCATCCTAAACCCTCGATCTTGTAAACAACCAATGTATA
CAAAATCAAACACAGAATCGAGA
   
Sequence Name 53-K030753-022-535-G10C-CV10
GenBank Accession LN508048 [GenBank]
Sequencing primer gene specific primer, CV10: 5'-TTCTCGATTCTGTGTTTGATTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K030753-022-535-G10C-CV10
AGATCGAGGGTTAGGATGATCGTGTTCGTTCGATTTAGCAATCATTTATCGATTCTGTGT
TTGATTTCGTGGAATTTGAAGTTTGTGTACAAGATCTAGAGTTGAAGATAATCCTTATGT
GTAATTTGGTTTCTTCGATGAATGTTACTCTTATTATAGAAGCTTTTGGTGCATATCTAT
GCTGGCTTCGATTGTTTGAGGGATTGAATAAGTTGTTGTGGTTATTTTACTGTGAAGGTT
TCGCGTCTCCCGTACATTTTCCAAACATTGGGTCTTGAATACGATGAGAAGGTTCTTCCA
TCGATTGGAAATGAGGTCTTGAAAGCTGTGGTTGCTAATTTCAATGCTGATCAGCTCCTC
ACTGAGCGTCCTCAAGTATCAGCACTTGTTCGTGATGCTCTTATCAAGCGTGCCAGGGAA
TTTAACATTGAGCTCTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11