Sequences from confirmation amplicon


Confirmation sequences for line 537B07 and hit At5g54610 are shown.
Line ID 537B07
Locus At5g54610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22184888
Primers used for PCR DR35 - 8474, show primer details
   
Sequence Name 28-K035540-022-537-B07C-DR35
GenBank Accession LN498747 [GenBank]
Sequencing primer gene specific primer, DR35: 5'-GTTTAAATGGTTGATCCGTGTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K035540-022-537-B07C-DR35
GGGATGTGATATGTAATTCAATATAACCACATACTATCAGAAATTAAATTGACCAAGAAG
TTGTTAGACCAAACACTCATTTATGTCATTCTTAAGTCTTAACATCATTAAACTCACATC
CTCTTTGAATAATTTATGTTATTATTCAGGTAGTAAAACAGCTGGTAAAATGCCTGTCAC
TGGACCGGAACATCCAGAACAAAAGTGGCATGACGGCCCTTGATGTCTTACGAGCCAGAG
GATCTCACATGAACAAAGAAATAGAGGAAATCATACAAATGTCCGGTGGTAAGACCGGAG
GCTCTCTATCCGGAATTCAGGAATGGTATATCTTCCTGAGGGAACCGGTCACTTTCAAGG
AACATTGCAAGACGCGAATTGCACGTTACAGGAGTCGCATATCAGACGGATCACGTAACG
CTCTTCTAGTAATAGCAGCTTTGATAATCTCAGCCACTTTTCAGACTGCAGCCCAACTGC
TAGACAAAGAAAAACTAGATAAAGTTAAAAAGAATGGTATGAGGTTTTCGGAATTCCAAC
TATGGGGATGCAATACGGTAGCGTTTTCCATAGCTATCCTATTTTCATTTATTCTCTTAC
CAGTTGGCAGAGCATATGAGTGGTGGTATTTCATTATTACGGTGCCCCTCGTCTTCTCTT
ATTTCCTTTTGATGTACATGATGCATGGTCTCTCATTCTTCTTCCTCATCATATATGAAG
GGGGCTTATTCCAAATGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGAC
   
Sequence Name 13-K035540-022-537-B07C-8409
GenBank Accession LN491944 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>13-K035540-022-537-B07C-8409
GAAGCCATTTACATTGAATATATCATTTGGAATAAGCCCCCTTCATATATGATGAGGAAG
AAGAATGAGAGACCATGCATCATGTACATCAAAAGGAAATAAGAGAAGACGAGGGGCACC
GTAATAATGAAATACCACCACTCATATGCTCTGCCAACTGGTAAGAGAATAAATGAAAAT
AGGATAGCTATGGAAAACGCTACCGTATTGCATCCCCATAGTTGGAATTCCGAAAACCTC
ATACCATTCTTTTTAACTTTATCTAGTTTTTCTTTGTCTAGCAGTTGGGCTGCAGTCTGA
AAAGTGGCTGAGATTATCAAAGCTGCTATTACTAGAAGAGCGTTACGTGATCCGTCTGAT
ATGCGACTCCTGTAACGTGCAATTCGCGTCTTGCAATGTTCCTTGAAAGTGACCGGTTCC
CTCAGGAAGATATACCATTCCTGAATTCCGGATAGAGAGCCTCCGGTCTTACCACCGGAC
ATTTGTATGATTTCCTCTATTTCTTTGTTCATGTGAGATCCTCTGGCTCGTAAGACATCA
AGGGCCGTCATGCCACTTTTGTTCTGGATGTTCCGGTCCAGTGACAGGCATTTTACCAGC
TGTTTTACTACCTGAATAATAACATAAATTATTCAAAGAGGATGTGAGTTTAATGATGTT
AAGACTTAAGAATGACATANATGAGTGTTTGGTCTAACAACTTCTTGGTCAATTTAATTT
CTGATAGTATGTGGTTATATTGAATTACATATTCACATCCCANATATACTAGCGTATAAT
TAAAGAG

Last Updated on 06.12.2018 16:11