Sequences from confirmation amplicon


Confirmation sequences for line 537E10 and hit At2g41630 are shown.
Line ID 537E10
Locus At2g41630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17355834
Primers used for PCR 09ht - 8474, show primer details
   
Sequence Name 48-K112689-0022-537-E10C-09ht
GenBank Accession LN506543 [GenBank]
Sequencing primer gene specific primer, 09ht: 5'-AAGAGGACAAGCCACGAACTATTA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K112689-0022-537-E10C-09ht
CCTCTTGATATAGCTTTGACATAAGTCAATCAAACTCGTGAACCTTGTGAATAGAAATAT
GCGTTATTGCCAATGGGGCGACAAAGAAGGAAATTGGCCGAGCAAAAGACTACATTGTTA
AGACATTGGGACTGGAGCCTGGTCAGTCTGTGGATTTAGGAACTATACACGCTGGTGATT
TCATGGTGAGTTTGTCTAAAACTAAAATTGTTGCTGATACCAGAACCTAGCTAGAGTGTG
CTAATTATGTTATGAAACAGTTGATTATGCTGGCTGCATTGACTACGTGGTTTCATAACT
CTGTATTTGATGAAATTGCAGAGAAGGTTCTGCTCCAACCTTGCAATGTCTAACCATGCG
GTGAAAGCTGCTCAGGAAGCTGTGCAAAAATCTGAGGAATTTGATATAAGGTACATTTCA
ATTATTTCCCTCAGTTCGGGACGGAGATAAACACAGCAGAATCTTAAAGATGTTTATTTT
GGGAAATTGCAGGAGGAGTCCTATATCAATAGCAGCTGATATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 16-K112688-0022-537-E10C-8409
GenBank Accession LN493497 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K112688-0022-537-E10C-8409
GCATTTCATTGAATATATCAGCTGCTATTGATATAGGACTCCTCCTGCAATTTCCCAAAA
TAAACATCTTTAAGATTCTGCTGTGTTTATCTCCGTCCCGAACTGAGGGAAATAATTGAA
ATGTACCTTATATCAAATTCCTCAGATTTTTGCACAGCTTCCTGAGCAGCTTTCACCGCA
TGGTTAGACATTGCAAGGTTGGAGCAGAACCTTCTCTGCAATTTCATCAAATACAGAGTT
ATGAAACCACGTAGTCAATGCAGCCAGCATAATCAACTGTTTCATAACATAATTAGCACA
CTCTAGCTAGGTTCTGGTATCAGCAACAATTTTAGTTTTAGACAAACTCACCATGAAATC
ACCAGCGTGTATAGTTCCTAAATCCACAGACTGACCAGGCTCCAGTCCCAATGTCTTAAC
AATGTAGTCTTTTGCTCGGCCAATTTCCTTCTTTGTCGCCCCATTGGCAATAACGCATAT
TTCTATTCACAAGGTTCACGAGTTTGATTGACTTATGTCAAAGCTATATCAAGAGGTAAC
AAGACTCTCTCAAATGAGAAATCTTTGAAATGTACCCTTAATAGTTCGTG

Last Updated on 06.12.2018 16:11