Sequences from confirmation amplicon


Confirmation sequences for line 537F08 and hit At4g27650 are shown.
Line ID 537F08
Locus At4g27650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13804749
Primers used for PCR NC52 - 8474, show primer details
   
Sequence Name 46-K101269-0022-537-F08C-8409
GenBank Accession LN505806 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K101269-0022-537-F08C-8409
ATTTACATTGAATAATCCTGTCGTGCACCAATATTATACGTGATTTATTCTCAATAATAG
GTCTCAGTTGTCTTCTTTCTGCTTCCAACAATAAGTGGCGATGAAACTGATCCTGGAGAC
ATGAAACAATCAAACGGTCTGTGTCATATATGTATGGGAAATTTGTAAGCCTGGTCCAGC
TGCAATTTGAAAAATAATAAACCACGTAAATTTAGAAAGTCTTTGAGTGACCAAAAAAAA
AACCTTTGTGAAGCCGGGACTTGCAACTACTGCACAGCGAACGACACTGAAGTCAACATG
TTTCACAAAGGCCTGTGATATTGCAATAGACTTTTAGATATGTGAAACTCTTTCACGCTT
T
   
Sequence Name 46-K101275-0022-537-F08C-NC52
GenBank Accession LN505807 [GenBank]
Sequencing primer gene specific primer, NC52: 5'-AAAAAGCGGAAAGAGTTTCACATA-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K101275-0022-537-F08C-NC52
GGCCTTTGTGAACATGTTGACTTCAGTGTCGTTCGCTGTGCAGTAGTTGCAAGTCCCGGC
TTCACAAAGGTTTTTTTTTTGGTCACTCAAAGACTTTCTAAATTTACGTGGTTTATTATT
TTTCAAATTGCAGCTGGACCAGGCTTACAAATTTCCCATACATATATAACACAAACCGTT
TGATTGTTTCATGTCTCCAGGATCAGTTTCATCGCCCCTTATTGTTGGAAGCAAAAAGAA
AACAACTGAAACCTATAATTGAGAATAAATCACGTATAAAATTGGTGCACGACAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAAAT
TTCC
Primers used for PCR CT37 - 3144, show primer details
   
Sequence Name 22-K105032-0022-537-F08C-CT37
GenBank Accession LN496106 [GenBank]
Sequencing primer gene specific primer, CT37: 5'-AGCAGAGAATCTCAGGCTGTTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K105032-0022-537-F08C-CT37
AAAATACAAACAAAGTAGAGTGAACCGTTAACACTGATAGCCACCATGAACATGAATCAT
AACATTACTAAAGTAGGTAACCTGGTCAATTCGGACATTGTTATACAAAAATTAAAAAAA
AAGTGTATGTTTCCTGCTAAAGAAATTATTAGGTTTATCCAGCAAATATCAACTCAAATC
GACAGTATAAATCTCAAATCAGTGTTCCACAACTTTAATAAATAAATTGCATGTTATATC
ATTTGACTCTGAGCACTCAATGTATTTTAGCATGGCAAAACAAAATTTTGACAGGCTTCA
TGAACAATTTCACAGAAATACTACCTATATCCAGAGTTTGTAAATATTCAATACCCGGAC
AATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAA
GGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
   
Sequence Name 38-K105033-0022-537-F08C-3144
GenBank Accession LN502973 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K105033-0022-537-F08C-3144
CTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCC
GGGGATCAGATTGTCCGGGTATTGAATATTTACAAACTCTGGATATAGGTAGTATTTCTG
TGAAATTGTTCATGAAGCCTGTCAAAATTTTGTTCTGCCATGCTAAAATACATTGAGTGC
TCAGAGTCAAATGATATAACATGCAATTTATTTATTAAAGTTGTGGAACACTGATTTGAG
ATTTATACTGTCGATCTGAGTTGATATCTGCTGGATAAACCTAATAATTTCTTTAGCAGG
AAACATACACTTTTTTTTTAATTTTTGTATAACAATGTCCGAATTGACCAGGTTACCTAC
TTTAGTAATGTTATGATTCATGTTCATGGTGGCTATCAGTGTTAACGGTTCACTCTACTT
TGTTTGTATTTTTTTTTGCTTATTCACTTCTAAACTAAACAGCC

Last Updated on 06.12.2018 16:11