Sequences from confirmation amplicon


Confirmation sequences for line 537G03 and hit At4g02610 are shown.
Line ID 537G03
Locus At4g02610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:1148434
Primers used for PCR 64ra - 8474, show primer details
   
Sequence Name 40-K115914-0022-537-G03C-64ra
GenBank Accession LN503826 [GenBank]
Sequencing primer gene specific primer, 64ra: 5'-CTCTCTTTCTTGTCATAGGCTGCT-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K115914-0022-537-G03C-64ra
AACACTTTACTCATTATCTCTATGCTTAAAGAGGTGTGTGTGGTTTGTTTTGTTTACTTA
CAGCCTCTCTTAGTTGTTTAGATTACATATGATGAAATTACAACTCTCATTGTTGAGTAG
ATAGTGGATTTTTTTTTGTTTTGGTTTTCTTACAAAGGGACACTTTTTATTCCAAAATTT
CTTCAGGTTATTCCTCAGTTATCTTGTCCAATTGCATTGTTTACATATTACAATCCAATC
CTGCGACGAGGAGTAGAGAACTACATGACTGTTATAAAGAATGCTGGAGTTCATGGTACA
TTGACATTTTCAATTTTTCTCCCAAGTAAAATACTTATATGCTGTTTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCC
   
Sequence Name 14-K115913-0022-537-G03C-8409
GenBank Accession LN492652 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K115913-0022-537-G03C-8409
ATCCTGAAACAGCATATAAGTATTTTACTTGGGAGAAAAATTGAAAATGTCAATGTACCA
TGAACTCCAGCATTCTTTATAACAGTCATGTAGTTCTCTACTCCTCGTCGCAGGATTGGA
TTGTAATATGTAAACAATGCAATTGGACAAGATAACTGAGGAATAACCTGAAGAAATTTT
GGAATAAAAAGTGTCCCTTTGTAAGAAAACCAAAACAAAAAAAAATCCACTATCTACTCA
ACAATGAGAGTTGTAATTTCATCATATGTAATCTAAACAACTAAGAGAGGCTGTAAGTAA
ACAAAACAAACCACACACACCTCTTTAAGCATAGAGATAATGGAGTTAAAGTTGGTTCCT
TTAAGTAACGAACGTCTCGCAGCAGCCTATGACAG

Last Updated on 06.12.2018 16:11