Sequences from confirmation amplicon


Confirmation sequences for line 540D01 and hit At4g27440 are shown.
Line ID 540D01
Locus At4g27440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13726866
Primers used for PCR 21ys - 8474, show primer details
   
Sequence Name 40-K122635-0022-540-D01C-21ys
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 21ys: 5'-TGCCTAAAGACAGCTACACAGTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K122635-0022-540-D01C-21ys
TTTGTTGATAATTTCGGAGAACGAGACGCCTCTCGATGTTTTGGTCTGCAATGCTGCGGT
TTATTTCCCGACAGCTAAAGAGCCTACTTACAGTGCTGAAGGGTTTGAGCTTAGTGTTGC
GACGAACCATTTGGGACATTTTCTTCTCGCAAGGTTGTTGCTTGATGACTTGAAGAAATC
TGATTACCCTTCAAAGCGTCTCATCATCGTCGGATCCATTACCGGTATTGTCTAGAATCT
GTATAACCCTACATTGCAAGATAGAGAATGATTTTGATCATTGTTATGTTTGTTTGGTTT
AGGGAACACGAATACATTGGCGGGTAATGTACCACCGAAGGCGAATCTCGGTGATTTGAG
GGGTTTAGCCGGCGGATTAAACGGTTTAAACAGCTCAGCTATGATTGATGGAGGAGATTT
CGACGGTGCAAAGGCTTACAAAGACAGTAAAGTCTGCAATATGTTGACAATGCAAGAGTT
TCACAGGCGTTTCCATGAAGAAACTGGAGTCACTTTCGCTTCGCTTTACCCCGGTTGCAT
CGCCTCCACAGGTTTATTCCGAGAGCACATTCCTCTCTTCCGTGCCCTCTTCCCTCCCTT
TCAGAAGTACATCACTAAAGGATATGTCTCCGAAACAGAGTCAGGCAAAAGACTTGCTCA
GGTAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTT
   
Sequence Name 16-K122634-0022-540-D01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K122634-0022-540-D01C-8409
CATTTCATTGAATATATTACCTGAGCAAGTCTTTTGCCTGACTCTGTTTCGGAGACATAT
CCTTTAGTGATGTACTTCTGAAAGGGAGGGAAGAGGGCACGGAAGAGAGGAATGTGCTCT
CGGAATAAACCTGTGGAGGCGATGCAACCGGGGTAAAGCGAAGCGAAAGTGACTCCAGTT
TCTTCATGGAAACGCCTGTGAAACTCTTGCATTGTCAACATATTGCAGACTTTACTGTCT
TTGTAAGCCTTTGCACCGTCGAAATCTCCTCCATCAATCATAGCTGAGCTGTTTAAACCG
TTTAATCCGCCGGCTAAACCCCTCAAATCACCGAGATTCGCCTTCGGTGGTACATTACCC
GCCAATGTATTCGTGTTCCCTAAACCAAACAAACATAACAATGATCAAAATCATTCTCTA
TCTTGCAATGTAGGGTTATACAGATTCTAGACAATACCGGTAATGGATCCGACGATGATG
AGACGCTTTGAAGGGTAATCAGATTTCTTCAAGTCATCAAGCAACAACCTTGCGAGAAGA
AAATGTCCCAAATGGTTCGTCGCAACACTAAGCTCAAACCCTTCAGCACTGTAAGTAGGC
TCTTTAGCTGTCGGGAAATAAACCGCAGCATTGCAGACCAAAACATCGAGAGGCGTCTCT
GTTCTCCTGAAATTATCAACAAACTGTCTCACGCTGTCCAACGAGGCTAAGTCTAAATGC
ATCACTGTGTAGCTGTCTTTAGGC

Last Updated on 06.12.2018 16:11