Sequences from confirmation amplicon


Confirmation sequences for line 540G02 and hit At5g02490 are shown.
Line ID 540G02
Locus At5g02490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:550844
Primers used for PCR AQ01 - 8474, show primer details
   
Sequence Name 28-K106287-0022-540-G02C-AQ01
GenBank Accession LN498857 [GenBank]
Sequencing primer gene specific primer, AQ01: 5'-CTCCAACTCCTTCATCTTGTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K106287-0022-540-G02C-AQ01
CATTGGTACATCTAGCCATTGGATTGCTTCCTCAATAGAGTCCTCAACCTTCTTCTTGTC
TGCAGCTGGAAGCTTCTCACCGATCTTCTCATCACGGATGGTGTTCCTCATGTTGTAAGC
GTAGTTCTCAAGAGCGTTCTTGGCCTCGACCTTCTTCTTGTGCTCCTCATCCTCAGACTT
GTACTTCTCCGCTTCTTGAACCATCTTCTCAATATCCTCTTTAGACAAACGACCCTTGTC
ATTAGTGATTGTGATCTTGTTCTTCTTTCCAGTGGTCTTGTCCTCAGCAGAGACGTTGAG
GATACCATTGGCGTCAATGTCGAAGCAGACAGTGATTTGAGGGACACCTCGTGGAGCAGG
ATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTAT
GATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGT
AGATGTCCGCACGNTTATTA
   
Sequence Name 28-K106286-0022-540-G02C-8409
GenBank Accession LN498856 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K106286-0022-540-G02C-8409
GGAATGAGCCTTTACATTGAATATATCCTGCTCCCGAGGTGTCCCTCAAATCACTGTCTG
CTTCGACATTGACGCCAATGGTATCCTCAACGTCTCTGCTGAGGACAAGACCACTGGAAA
GAAGAACAAGATCACAATCACTAATGACAAGGGTCGTTTGTCTAAAGAGGATATTGAGAA
GATGGTTCAAGAAGCGGAGAAGTACAAGTCTGAGGATGAGGAGCACAAGAAGAAGGTCGA
GGCCAAGAACGCTCTTGAGAACTACGCTTACAACATGAGGAACACCATCCGTGATGAGAA
GATCGGTGAGAAGCTTCCAGCTGCAGACAAGAAGAAGGTTGAGGACTCTATTGAGGAAGC
AATCCAATGGCTAGATGGTAACCAATTGGGTGAAGCTGACGAGTTTGAAGACAAGATGAA
GAAGTTGGAG

Last Updated on 06.12.2018 16:11