Sequences from confirmation amplicon


Confirmation sequences for line 540H12 and hit At5g49460 are shown.
Line ID 540H12
Locus At5g49460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20057621
Primers used for PCR KR53 - 8474, show primer details
   
Sequence Name 03-K100810-0022-540-H12C-8409
GenBank Accession LN487399 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K100810-0022-540-H12C-8409
GCCATATAATTTCTGAATCTGAATTGTAATTGGTATTAATAACATTGGTTGCTTGTTTCA
GAATCTCACACCTTATGAATTTGTTGAGGGAATGAAGAAAAAGGGAATCCGAGTCCCCGG
GATTGGACACAGGTAGAAAACAGAACATTGTACCAAAGAAACAGGCAAATCCACACAGCT
TGTCTTGTCTGAAAACTGTTTTCTTCTTTATGGTAGGATCAAGAGCAGAGACAACAGAGA
CAAAAGAGTGGAGCTTCTTCAGAAATTTGCTCGGTCCAACTTCCCATCAGTGAAGTACAT
GGAGTACGCAGTGACAGTGGAGACATACACGCTCTCAAAGGCAAACAACCTCGTACTCAA
CGTTGATGGAGCCATTGGATCTCTCTTCTTGGACCTTCTAGCTGGAAGTGGGATGTTCAC
TAAACAAGAGATTGACGAGATTGTTCAGATCGGTTATCTCAAC
   
Sequence Name 03-K100824-0022-540-H12C-KR53
GenBank Accession LN487401 [GenBank]
Sequencing primer gene specific primer, KR53: 5'-AACAAACAGACCGTTGAGATAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K100824-0022-540-H12C-KR53
TGACATCCCACTTCCAGCTAGAAGGTCCAAGAAGAGAGATCCAATGGCTCCATCAACGTT
GAGTACGAGGTTGTTTGCCTTTGAGAGCGTGTATGTCTCCACTGTCACTGCGTACTCCAT
GTACTTCACTGATGGGAAGTTGGACCGAGCAAATTTCTGAAGAAGCTCCACTCTTTTGTC
TCTGTTGTCTCTGCTCTTGATCCTACCATAAAGAAGAAAACAGTTTTCAGACAAGACAAG
CTGTGTGGATTTGCCTGTTTCTTTGGTACAATGTTCTGTTTTCTACCTGTGTCCAATCCC
GGGGACTCGGATTCCCTTTTTCTTCATTCCCTCAACAAATTCATAAGGTGTGAGATTCTG
AAACAAGCAACCAATGTTATTAATACCAATTACAATTCAGATTCAGAAATTTATATNGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG

Last Updated on 06.12.2018 16:11