Sequences from confirmation amplicon


Confirmation sequences for line 541A03 and hit At3g60600 are shown.
Line ID 541A03
Locus At3g60600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22401869
Primers used for PCR 49fr - 8474, show primer details
   
Sequence Name 25-K111563-0022-541-A03C-49fr
GenBank Accession LN497505 [GenBank]
Sequencing primer gene specific primer, 49fr: 5'-CATGGTTCTGAATTTTCGGTTAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K111563-0022-541-A03C-49fr
ATATTTATATGTAGATTTTCATGTTTAATTTTAGTTTGAGAGATTTATCGTGGACAACAA
GGCTGGACATCAAGAAAACACATCTGAGGTATTTTTCACAATTCTTCCAAGTTTGTCACC
GTCAAAACCATGTGACATCATCTTTTGCTTTTGCTCTGTTTAAAACTGATTTTTGGAGGT
AGCTTTACTGGAAAGTTGCCTTTTAGGCTGATTTACTAGGTTGAGAATCTTTCTTTATGT
TGATTACTTAAAAGGTTCACTACATTTTTAGAAGCCTGTCCAGTGCTCAAGCCGTCCATT
AATTGTCTTTTTGTGCACCAAATCCTCGTAGATGTTATTTTGACACAGTTACCACTTACT
ATACACTATTTTGCTTCAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAA
TCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACC
AATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAATTATA
   
Sequence Name 09-K111556w-0022-541-A03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K111556w-0022-541-A03C-8409
CATTTCATTGAATAATCCTGACTGAAGCAAATAGTGTATAGTAAGTGGTAACTGTGTCAA
AATAACATCTACGAGGATTTGGTGCACAAAAAGACAATTAATGGACGGCTTGAGCACTGG
ACAGGCTTCTAAAAATGTAGTGAACCTTTTAAGTAATCAACATAAAGAAAGATTCTCAAC
CTAGTAAATCAGCCTAAAAGGCAACTTTCCAGTAAAGCTACCTCCAAAAATCAGTTTTAA
ACAGAGCAAAAGCAAAAGATGATGTCACATGGTTTTGACGGTGACAAACTTGGAAGAATT
GTGAAAAATACCTCAGATGTGTTTTCTTGATGTCCAGCCTTGTTGTCCACGATAAATCTC
TCAAACTAAAATTAAACATGAAAATCTACATATAAATATGTCTACCAGAGAAACTGAAAT
CTAAACTTAAGTTAACATGTACTAACCGAAAATTGGAAACCAT

Last Updated on 06.12.2018 16:11