Sequences from confirmation amplicon


Confirmation sequences for line 541D11 and hit At5g65480 are shown.
Line ID 541D11
Locus At5g65480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26177211
Primers used for PCR CH10 - 8474, show primer details
   
Sequence Name 49-K106741-0022-541-D11C-CH10
GenBank Accession LN506854 [GenBank]
Sequencing primer gene specific primer, CH10: 5'-ACACCCACATGAAAAATATCCAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K106741-0022-541-D11C-CH10
CTTCTGTTGCTAGGTCTCCATCAGCATCATCTTTGTCCATTAGTAGATTCTTGAGATATG
ANCTTGGTGTTTTGTTGTCACTCGCTTCAAACAGAAAATCAAAAGAAGTCTCCAGAAGCT
ATATCCACGAACGAATCTTGCTTCTTCTTTTTTTTTATTTTCCTTTTCTTTTTTTTTTAG
ATTATTCGATGCTTAAATCTCGATACTGAAACAACAGAGAACTAGTCTTATTCTTCCCAA
TCTCCAATTTATAGCTTCCCCATAGACGACAGCCACTGTTCCATGTTCTTCCCATCTGCA
GTTTACATGTCATCAGAACAAAGAACAAATCAATTTACAAACAAAAGACTCAAACCAATT
ACTTAAGTTCTTAAAAGAACATGCAGATAATTTACATGATAGCTATTAATGAATGATGAT
GATAATGGAAACAAACATTACCAGGAGCAGCAGGAGAGAAGAAGCCATTCGGCAACTGGT
TATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAATGAGTA
TGATGGTCAATATGGAGAAAAAAAAAGAGTAATTACCAATTTTTTTTCATTTCAAAAATG
TTAAAGTCCGCGCCGTTATAAAAAA
   
Sequence Name 33-K106740-0022-541-D11C-8409
GenBank Accession LN501013 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K106740-0022-541-D11C-8409
ATTTACAATTGAATATATAACCAGTTGCCGAATGGCTTCTTCTCTCCCGCTGCTCCTGGT
AATGTTTGTTTCCATTATCATCATCATTCCTTAATAGCTATCATGTAAATTATCTGCATG
TTCTTTTAAGAACTTAAGTAATTGGTTTGAGTCTTTTGTTTGTAAATTGATTTGTTCTTT
GTTCTGATGACATGTAAACTGCAGATGGGAAGAACATGGAACAGTGGCTGTCGTCTATGG
GGAAGCTATAAATTGGAGATTGGGAAGAATAAGACTAGTTCTCTGTTGTTTCAGTATCGA
GATTTAAGCATCGAATAATCTAAAAAAAAAAGAAAAGGAAAATAAAAAAAAAGAAGAAGC
AAGATTCGTTCGTGGATATAGCTTCTGGAGACTTCTTTTGATTTTCTGTTTGAAGCGAGT
GACAACAAAACACCAAGTCATATCTCAGGAATCAACTACTGGACAAAGATGAGGTTTATG
GATACCTAGCAAGTGGAACATGACCACGATATGATTTGAGTATTGGATATTTTTCAAGAG
GGTG

Last Updated on 06.12.2018 16:11