Sequences from confirmation amplicon


Confirmation sequences for line 543A09 and hit At5g18450 are shown.
Line ID 543A09
Locus At5g18450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6116149
Primers used for PCR HV04 - 8474, show primer details
   
Sequence Name 20-K031868-022-543-A09C-HV04
GenBank Accession LN495100 [GenBank]
Sequencing primer gene specific primer, HV04: 5'-GTGGTTCGAATCTTTGTTGGTTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K031868-022-543-A09C-HV04
CCAGATCAGAGACCGTTATGCTACGACTAGTTATGGCGGTGAAGGCGGTGGTGGCTCTAC
GTTAACGTTTTCGACCAATTTGAAACCAAAGAATTTGATGAGTCAGAATTATGGATTATA
CAATGGAGCTTGGTCTAGGTTTCTTGTGGGGCAAGAAAAGAAGACGGAACATGACGTGTC
ATCGTCGTGTGGATCGTCGGACAACAAGGAGAGTATGTTGGTTCCTAGTTGCGGCGGAGA
GAGGATGCATAGGCCGGAGTTGGAAGAGCGAACAGGATATTTGGAAATGGATGATCTTTT
GGAGATTGATGATTTAGGTTTGTTGATTGGCAAAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGA
TAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTA
ATTCGGAAATCTTTATTTCGACGTGTCTACATTCACTCCCAATGG
   
Sequence Name 19-K031868-022-543-A09C-8409
GenBank Accession LN494648 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K031868-022-543-A09C-8409
GCCATTTTTGCCATCAACAAACCTAAATCATCAATCTCCAAAGATCATCCATTTCCAAAT
ATCCTGTTCGCTCTTCCAACTCCGGCCTATGCATCCTCTCTCCGCCGCAACTAGGAACCA
ACATACTCTCCTTGTTGTCCGACGATCCACACGACGATGACACGTCATGTTCCGTCTTCT
TTTCTTGCCCCACAAGAAACCTAGACCAAGCTCCATTGTATAATCCATAATTCTGACTCA
TCAAATTCTTTGGTTTCAAATTGGTCGAAAACGTTAACGTAGAGCCACCACCGCCTTCAC
CGCCATAACTAGTCGTAGCATTAACGGTCTCTTGATCTTGTGAATCACTTTGTTTAGGTA
ATAACCAACAAAGNATTCGAACCACA
Primers used for PCR FS17 - 8474, show primer details
   
Sequence Name 46-K118546w-0022-543-A09C-FS17
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, FS17: 5'-GGTTTATGTGATGGCCTTAGTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118546w-0022-543-A09C-FS17
ATGTTTGATGTTTAATTTGTAAGACCGGACACATGAGCAGTATAAGGATTTGTGATATTA
ATCTATATGTGTTTTCGTTTTGAAGATATGTCCGCAGGAAACAGTAAGATAAATAATCTG
ATACAAGGAACATGAGAGTAAAAATAGTGTCACATTCGAAACTTGCTTCATCTCTATTAA
TTTGTTTTTACAAAACGTGTAAGAAAATTCACATTATGCCCAAACCTATTTACACACATA
AGCACTACATGCAAGGAGAAAACAAAAAATCCCACGATTGATGTATAACAAATAAACAAT
CCTAAGTCAAATATAAGAAATATGTATGATAAATAATAGTAATAAATAAAAACTCTCAGA
ACCAATTCCATGGATGTTGAAACTCTTCACAACACCAATTCTTGAAATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
ATTAT
   
Sequence Name 26-K118545w-0022-543-A09C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K118545w-0022-543-A09C-8409
ATTTACATTGAATATTTCAAGAATTGGTGTTGTGAAGAGTTTCAACATCCATGGAATTGG
TTCTGAGAGTTTTTATTTATTACTATTATTTATCATACATATTTCTTATATTTGACTTAG
GATTGTTTATTTGTTATACATCAATCGTGGGATTTTTTGTTTTCTCCTTGCATGTAGTGC
TTATGTGTGTAAATAGGTTTGGGCATAATGTGAATTTTCTTACACGTTTTGTAAAAACAA
ATTAATAGAGATGAAGCAAGTTTCGAATGTGACACTATTTTTACTCTCATGTTCCTTGTA
TCAGATTATTTATCTTACTGTTTCCTGCGGACATATCTTCAAAACGAAAACACATATAGA
TTAATATCACAAATCCTTATACTGCTCATGTGTCCGGTCTTACAAATTTAAACATCAAAC
ATCTTCATCACATGAGCTATCATCGCCATCCACATCATCGCCATCACTAAGGCCATAACA
TAAACC

Last Updated on 06.12.2018 16:11